Tuesday, April 29, 2025
4/29/2025
Multistage LC-MSn for automated glycan isomer assignment of glycopeptides - N- and O-linked glycosylation are some of the most common, abundant, and biologically important posttranslational modifications of proteins, yet they are also some of the most difficult to study. The dominant analytical platform for the study of glycosylation is mass spectrometry, yet this platform on its own is incomplete, because it cannot elucidate glycan topology and linkage stereochemistry. This project will advance the use of mass spectrometry for glycan structure determination, especially for the case of O-linked glycans, by studying the ion propensities of various structural motifs in multi-stage ion trap mass spectrometry, and by building new software for structure inference from such mass spectra.
HHS’ Tracking Accountability in Government Grants System (TAGGS) website provides access to detailed descriptions of grants, loans, aggregated direct payments and other types of financial assistance awarded by the HHS Operating Divisions (OPDIVs) and the Office of the Secretary Staff Divisions (STAFFDIVs).