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Comprehensive Glycoproteomic Tool Development for Cancer Biomarkers

Award Number: R41GM112750
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/22/2014
PERIOD OF PERFORMANCE END DATE: 03/31/2017

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Comprehensive Glycoproteomic Tool Development for Cancer Biomarkers - DESCRIPTION (provided by applicant): This project addresses the need for better methods for deciphering the glycosylation of proteins in clinical samples. Glycosylation is an important modifier of protein structure and function and contributes to disease processes. But we currently know little about the glycosylation of most proteins. The current methods for probing glycans on proteins are not suitable for meeting this need, as they require much material and many processing steps. Here we propose and practical approach to probing protein glycosylation that will provide: 1) the ability to obtain structural and compositional information with limited sample usage; 2) the ability to precisely compare glycan levels between samples; and 3) ready translation into a clinical assay. We will achieve this goal through novel informatics techniques that facilitate the combined use of mass spectrometry (MS) and lectin binding for studying glycans. Phase II will focus on glycoprotein biomarkers of pancreatic cancer. MS provides the monosaccharide compositions of glycans and some sequence information, but it leaves ambiguities about sequence or linkage variants. Likewise, lectins can give precise measurements of specific structures using small amounts of sample, but they do not provide a complete picture of each glycan. We predict that quantitatively integrating the two types of information will give more accurate information than either method alone. We will quantitatively link lectin experiments to MS experiments using the common language of motifs - substructures of glycans. In Aim 1, we will develop an algorithm for identifying what glycan motifs are most likely present in a sample based on lectin binding. In Aim 2, we will develop tools for integrating lectin and MS data and will use the method to characterize and compare the glycans of three different purified glycoproteins. We will determine whether the linking of MS and lectin data provides more complete information than either method alone, with limited sample consumption and the ability to make precise comparisons between samples.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2017 ( Subtotal = $0 )
2017	2015	PROTEIN METRICS INC.	1622 SAN CARLOS AVE, SUITE C	SAN CARLOS	CA	94070	SAN MATEO	USA	Biomedical Research and Research Training	000	2	8/10/2017	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2015 ( Subtotal = $342,930 )
2015	2015	PROTEIN METRICS INC	POST OFFICE BOX 414	SAN CARLOS	CA	94070	SAN MATEO	USA	Biomedical Research and Research Training	002	2	9/7/2015	NON-COMPETING CONTINUATION	$342,930
2015	2014	PROTEIN METRICS INC	POST OFFICE BOX 414	SAN CARLOS	CA	94070	SAN MATEO	USA	Biomedical Research and Research Training	000	1	10/21/2014	NEW	-$342,930
2015	2014	PROTEIN METRICS INC	POST OFFICE BOX 414	SAN CARLOS	CA	94070	SAN MATEO	USA	Biomedical Research and Research Training	001	1	10/22/2014	NEW	$342,930
														Subtotal = $342,930

Issue Date FY: 2014 ( Subtotal = $342,930 )
2014	2014	PROTEIN METRICS INC	POST OFFICE BOX 414	SAN CARLOS	CA	94070	SAN MATEO	USA	Biomedical Research and Research Training	000	1	9/22/2014	NEW	$342,930
														Subtotal = $342,930

Grand Total All Awards = $685,860

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Comprehensive Glycoproteomic Tool Development for Cancer Biomarkers

Award Number: R41GM112750

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/22/2014

PERIOD OF PERFORMANCE END DATE: 03/31/2017

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