Sunday, August 17, 2025 8/17/2025

Molecular Mechanisms Underlying TRPM3 Regulation in the Cell Membrane

Award Number: R35GM160009
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/04/2025
PERIOD OF PERFORMANCE END DATE: 06/30/2030

Group Awards By:

View Award Description

Molecular Mechanisms Underlying TRPM3 Regulation in the Cell Membrane - SUMMARY Membrane proteins and lipids are organized into signaling domains in the cell membrane that are hundreds of nanometers in size. These signaling domains are essential for the efficiency and specificity required for cell signaling. The long-term vision of my research program is to determine the molecular compositions and structures of these membrane protein signaling complexes and understand how their organization results in their physiological function. This overall vision will be fulfilled through two independent tasks. One is to determine the molecular mechanisms underlying individual components in a signaling pathway. The other is to directly study the complex as a whole to understand how different components are assembled with each other. In the next five years, my goal is to exercise these two tasks on a specific membrane protein ─ the TRPM3 ion channel. TRPM3 plays a critical role in nociceptor neurons and mediates inflammatory hyperalgesia and pain sensation. The activity of TRPM3 is tightly regulated by the G protein-coupled receptor (GPCR) signaling pathway. Specifically, we will determine the structures and activities of individual TRPM3 channels in isolation and in cell membranes to understand the molecular details governing its transition between closed, open, and desensitized states. We will also determine the compositions and structures of the TRPM3 signaling complex in the cell membrane to understand how the organization of lipid and protein molecules contributes to TRPM3 signaling efficacy. We will use electrophysiological recordings in cells and in reconstituted membranes to isolate the contributions from individual protein and lipid partners. The results from this proposal will not only advance our molecular understanding of the TRPM3 function but also contribute to the emerging field of integrative structural biology, where we study the structure of signaling complexes in the native environment using a multitude of atomistic and molecular approaches. Our efforts will also lead to the development of new methods to study TRPM3 ion channels in their native environments that are broadly applicable to other membrane proteins and signaling pathways.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $410,601 )
2025	2025	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	000	1	8/4/2025	NEW	$410,601
														Subtotal = $410,601

Grand Total All Awards = $410,601

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Molecular Mechanisms Underlying TRPM3 Regulation in the Cell Membrane

Award Number: R35GM160009

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/04/2025

PERIOD OF PERFORMANCE END DATE: 06/30/2030

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer