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Sensor-Like LC-MS Platform for Rapid Online Monitoring of Metabolites in Biological Systems

Award Number: R35GM154693
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/15/2024
PERIOD OF PERFORMANCE END DATE: 06/30/2029

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Sensor-Like LC-MS Platform for Rapid Online Monitoring of Metabolites in Biological Systems - Project Summary Our research program focuses on the development of analytical tools to enhance the characterization of complex biological systems. For rapid, real-time analysis of these systems, sensors based on spectroscopic, electrochemical, and mass spectrometric detection have been widely utilized. However, the capability of these approaches to simultaneously monitor multiple analytes suffers without the use of chemical separations. To achieve sensor-like performance with a separations-based platform, this project focuses on the creation of a liquid chromatography-mass spectrometry (LC-MS) platform for near-universal, real-time online measurement of targeted small molecule metabolites. High-throughput gradient LC separations at capillary-scale flow rates will be achieved through system miniaturization and improved microfluidic mixing combined with a droplet-based injection approach. Improved analyte selectivity and sensitivity for targeted metabolites will be achieved with an online benzoyl chloride derivatization device. To improve the temporal resolution of sampling, the derivatization technique will be adapted to a segmented flow droplet format. Dual column re-equilibration will further increase throughput by 20%, achieving an overall method cycle time of 10-15 s. By combining these fundamental advances in separation science, a transformational measurement platform will be achieved. The system will be used to specifically probe: (1) neurotransmitter release from organoid cell models of traumatic brain injury, (2) polyamine secretion during bacterial biofilm formation, and (3) cell culture media nutrient depletion observed during therapeutic antibody manufacturing.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $375,000 )
2025	2025	ROWAN UNIVERSITY	201 MULLICA HILL RD	GLASSBORO	NJ	08028	GLOUCESTER	USA	Biomedical Research and Research Training	000	2	6/26/2025	NON-COMPETING CONTINUATION	$375,000
														Subtotal = $375,000

Issue Date FY: 2024 ( Subtotal = $375,000 )
2024	2024	ROWAN UNIVERSITY	201 MULLICA HILL RD	GLASSBORO	NJ	08028	GLOUCESTER	USA	Biomedical Research and Research Training	000	1	9/9/2024	NEW	$375,000
														Subtotal = $375,000

Grand Total All Awards = $750,000

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Sensor-Like LC-MS Platform for Rapid Online Monitoring of Metabolites in Biological Systems

Award Number: R35GM154693

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/15/2024

PERIOD OF PERFORMANCE END DATE: 06/30/2029

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