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Advanced approaches for biomedical measurements using time-resolved and micro-flow cytometry

Award Number: R35GM152076
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 01/01/2024
PERIOD OF PERFORMANCE END DATE: 12/31/2028

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Advanced approaches for biomedical measurements using time-resolved and micro-flow cytometry - Project Summary The objective of this MIRA is to expand research and development within the field of high-throughput, single cell analysis to address challenges with how cells are screened, how data are collected and processed, and how effectively different technologies quantitatively inform biomedicine. For many years (and under the auspices of a current R01), our laboratory's main efforts have focused on the development of TRFC as a unique form of cytometry that captures fluorescence lifetimes and/or decay kinetic profiles from fluorescently labeled particles and cells so as to sort and/or analyze cell-to-cell differences with the lifetime as a distinguishing parameter. The main goal for this MIRA will be to exploit the state-of-the-art in time-resolved flow cytometry (TRFC) and take this area of research to new heights. That is, we will seek to advance TRFC so that it supports multi-color cytometry, microfluidic-based cytometry, viscoelastic cytometry, optical redox cytometry, and potentially imaging cytometry. The main application to be studied will be changes in metabolic pathways of cells by exploiting autofluorescence lifetimes of free and bound NAD(P)H and FAD. We will focus on predictive and metabolic models of the dynamic changes in cellular respiration for cultured breast cancer cells treated with tamoxifen. With a flexible MIRA we will seek out different ways that TRFC can inform changes in cells when treated and whey they exhibit chemoresistance. One way to accomplish this will be through quantitative optical redox measurements with a TRFC system. Additionally, in a tangentially field of cytometry, this work will develop a new area of research involving sorting and separation of exosomes and microvesicles from cells. We will build on our existing computational fluid dynamic models to do so. In general, the development of advanced, sensitive, and time-dependent cytometry system is impacting beyond the metabolic and exosome applications proposed. Accordingly, this work is the first step toward evaluating the benefits, demonstrating the quantitative nature, and setting the stage for broad use across many more cytometric applications.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $327,974 )
2026	2026	NEW MEXICO STATE UNIVERSITY	1050 STEWART ST.	LAS CRUCES	NM	88003	DONA ANA	USA	Biomedical Research and Research Training	000	3	1/21/2026	NON-COMPETING CONTINUATION	$327,974
														Subtotal = $327,974

Issue Date FY: 2025 ( Subtotal = $364,416 )
2025	2025	NEW MEXICO STATE UNIVERSITY	1050 STEWART ST.	LAS CRUCES	NM	88003	DONA ANA	USA	Biomedical Research and Research Training	002	2	8/12/2025	NON-COMPETING CONTINUATION	$36,442
2025	2025	NEW MEXICO STATE UNIVERSITY	1050 STEWART ST.	LAS CRUCES	NM	88003	DONA ANA	USA	Biomedical Research and Research Training	003	2	8/15/2025	NON-COMPETING CONTINUATION	$0
2025	2025	NEW MEXICO STATE UNIVERSITY	1050 STEWART ST.	LAS CRUCES	NM	88003	DONA ANA	USA	Biomedical Research and Research Training	001	2	12/17/2024	NON-COMPETING CONTINUATION	$327,974
2025	2024	NEW MEXICO STATE UNIVERSITY	1050 STEWART ST.	LAS CRUCES	NM	88003	DONA ANA	USA	Biomedical Research and Research Training	000	1	11/21/2024	NEW	$0
														Subtotal = $364,416

Issue Date FY: 2024 ( Subtotal = $364,666 )
2024	2024	NEW MEXICO STATE UNIVERSITY	1050 STEWART ST.	LAS CRUCES	NM	88003	DONA ANA	USA	Biomedical Research and Research Training	000	1	12/26/2023	NEW	$364,666
														Subtotal = $364,666

Grand Total All Awards = $1,057,056

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Advanced approaches for biomedical measurements using time-resolved and micro-flow cytometry

Award Number: R35GM152076

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 01/01/2024

PERIOD OF PERFORMANCE END DATE: 12/31/2028

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