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Learning How to Give Casual Explanations for Large Scale Virtual and Morphological Pharmacology

Award Number: R35GM151129
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/20/2023
PERIOD OF PERFORMANCE END DATE: 08/31/2028

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Learning How to Give Casual Explanations for Large Scale Virtual and Morphological Pharmacology - To unravel the complexity of biological systems researchers have traditionally studied reductive model systems like cultured cells or simple molecular simulations. While these reductive model systems can be cheaper, easier, and/or more ethical to manipulate, findings in them may not translate to the biological systems of primary interest. This is especially important for drug-discovery, as late-stage failures result in enormous costs and long development timelines. Excitingly, recent advances in biotechnology and computing have made more complex model systems—including 3D organoids and large-scale virtual screening—more tractable. However, an emerging challenge is that standard statistical methods developed to analyze simple model systems are insufficient to analyze these more complex model systems. Complex model systems are inherently heterogeneous. The key statistical challenge is to leverage the higher dimensional readouts afforded by the new technologies to identify the causal mechanisms relevant for translation. When done properly, better statistical analysis can unlock the potential of new technology to better represent target biological systems with more precision and less bias. The overarching theme of my research program is to develop causal inference methods for complex model systems for pharmacology. Complex systems analyze in my group include morphological profiling, where robotic confocal microscopes with multiplexed fluorescent dyes are used to rapidly characterize the rich cellular morphological of individual cells, and large-scale virtual screening, where molecular simulations are used prioritize compounds from make-on-demand libraries containing tens of billions of molecules. We draw parallels across these distinct screening platforms, we develop and apply causal inference methods to better guide translatable discoveries. Project one: Account for spatial call-to-environment and cell-to-cell interactions in morphological profiling of organoids in 3D culture. Depending on the downstream application, spatial factors can either define or confound relevant biological responses. We will develop global and local models for cellular spatial factors and use them as statistical controls while avoiding selection bias to model the effects of chemical perturbations. Project two: Mapping bioactive chemical space for adaptive large-scale virtual screening. AI guided synthesis prediction is rapidly open new chemical spaces for virtual screening. However, it is not clear how to take advantage of the increased chemical diversity to best improve target specific or selectivity. We propose to train high-capacity deep-learning models to represent compounds based their compatibility with ligand binding sites. This chemical-space map will enable characterizing how perturbations to virtual screening binding sites and simulation methods effect the distribution of predicted ligands.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $381,484 )
2024	2024	REGENTS OF THE UNIVERSITY OF MICHIGAN	1109 GEDDES AVE, SUITE 3300	ANN ARBOR	MI	48109	WASHTENAW	USA	Biomedical Research and Research Training	000	2	9/6/2024	NON-COMPETING CONTINUATION	$381,484
														Subtotal = $381,484

Issue Date FY: 2023 ( Subtotal = $381,889 )
2023	2023	REGENTS OF THE UNIVERSITY OF MICHIGAN	1109 GEDDES AVE, SUITE 3300	ANN ARBOR	MI	48109	WASHTENAW	USA	Biomedical Research and Research Training	000	1	9/18/2023	NEW	$381,889
														Subtotal = $381,889

Grand Total All Awards = $763,373

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Learning How to Give Casual Explanations for Large Scale Virtual and Morphological Pharmacology

Award Number: R35GM151129

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/20/2023

PERIOD OF PERFORMANCE END DATE: 08/31/2028

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