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NanoSMS: single molecule secretome analysis for non-destructive cellular fingerprinting

Award Number: R35GM151115
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2023
PERIOD OF PERFORMANCE END DATE: 08/31/2028

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NanoSMS: single molecule secretome analysis for non-destructive cellular fingerprinting - Project Summary/Abstract Over the next 5 years, NanoSMS will be developed into a technology which offers the first look into the single cell secretome; with single molecule sensitivity. The development of this new technology is based on the integration of single cell encapsulation technologies with nanopore- based single molecule sensing. The collection of molecules released into the extracellular environment by single cells, termed the single cell secretome, offers a unique glimpse into the complexities of cellular life and cell-to-cell communication. The key features of the technology which will be developed at the end of the project period include (1) a massively parallel way to form droplets adhered onto a glass slide and containing a single cell, and (2) a nanopore-based approach for measuring secreted molecule originating from a single cell. Once the nanopores and related methodologies are developed, we aim to answer four key questions surrounding important biomedical issues. First, the nanopore will quantify single cell antibody production originating from human plasma cells which have been isolated from patients in various disease states including auto-immune disorders. Second, B cells and T cells will be co-incubated and stimulated with a single antigen to induce an immune response. The time lag to start generating antibodies as well as the factors which influence the timing of the immune response will be studied. Third, eosinophils co-incubated with a single cancer cell (or cancer-derived vesicles) will be investigated to provide insight into early cancer recognition mechanisms. Lastly, single cell secretome fingerprinting will be used to access the ability to discriminate between stem cell lineages in a non-destructive manner. Stem cell characterization is important for cell therapy (in which the mechanism of action is secreted molecules) as well as assessing cell state for regenerative medicine. Needless to say, the secretome plays a functional and yet obscured role in both normal and diseased cellular states. The application of NanoSMS (a nanopore-based approach towards secretome analysis) will provide a unique and new tool for single cell characterization.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $358,250 )
2024	2024	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	200 UNIVERSTY OFC BUILDING	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	000	2	9/19/2024	NON-COMPETING CONTINUATION	$358,250
														Subtotal = $358,250

Issue Date FY: 2023 ( Subtotal = $346,911 )
2023	2023	REGENTS OF THE UNIVERSITY OF CALIFORNIA AT RIVERSIDE	200 UNIVERSTY OFC BUILDING	RIVERSIDE	CA	92521	RIVERSIDE	USA	Biomedical Research and Research Training	000	1	8/23/2023	NEW	$346,911
														Subtotal = $346,911

Grand Total All Awards = $705,161

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NanoSMS: single molecule secretome analysis for non-destructive cellular fingerprinting

Award Number: R35GM151115

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2023

PERIOD OF PERFORMANCE END DATE: 08/31/2028

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