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Single cell quantification of translation control in early mouse development

Award Number: R35GM150667
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/01/2023
PERIOD OF PERFORMANCE END DATE: 06/30/2028

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Single cell quantification of translation control in early mouse development - The initial stages of vertebrate embryo development proceed in the absence of transcription. Consequently, protein abundance of preimplantation embryos is regulated by post-transcriptional gene expression programs. In particular, new protein synthesis is required for productive embryogenesis as inhibition of translation prevents activation of the zygotic genome and leads to arrested development. Despite this critical function, translational dynamics in single mammalian embryos have remained unexplored due to technological limitations. To address this critical gap and technological need, we propose to comprehensively and quantitatively determine the dynamics of translational control during early mouse development at single nucleotide and single embryo resolution. The proposed research will apply a transformative technology that my lab developed enabling the measurement of translation in single preimplantation embryos. In this study, we will determine allele-specific ribosome engagement of mRNAs and characterize the underlying mechanisms. The proposed study will be significant as it will be the first transcriptome-wide characterization of translational control in preimplantation mammalian development at single embryo resolution and will reveal how the early embryo executes specific gene expression programs to shape its proteome.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $389,230 )
2025	2025	UNIVERSITY OF TEXAS AT AUSTIN	110 INNER CAMPUS DR	AUSTIN	TX	78712	TRAVIS	USA	Biomedical Research and Research Training	000	3	6/10/2025	NON-COMPETING CONTINUATION	$389,230
														Subtotal = $389,230

Issue Date FY: 2024 ( Subtotal = $389,230 )
2024	2024	UNIVERSITY OF TEXAS AT AUSTIN	110 INNER CAMPUS DR	AUSTIN	TX	78712	TRAVIS	USA	Biomedical Research and Research Training	000	2	7/12/2024	NON-COMPETING CONTINUATION	$389,230
														Subtotal = $389,230

Issue Date FY: 2023 ( Subtotal = $389,230 )
2023	2023	UNIVERSITY OF TEXAS AT AUSTIN	110 INNER CAMPUS DR	AUSTIN	TX	78712	TRAVIS	USA	Biomedical Research and Research Training	000	1	7/21/2023	NEW	$389,230
														Subtotal = $389,230

Grand Total All Awards = $1,167,690

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Single cell quantification of translation control in early mouse development

Award Number: R35GM150667

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/01/2023

PERIOD OF PERFORMANCE END DATE: 06/30/2028

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