Friday, November 14, 2025 11/14/2025

Modeling transcriptional and post-transcriptional systems for regulating non-genetic heterogeneity in mammalian cells

Award Number: R35GM149531
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 06/01/2023
PERIOD OF PERFORMANCE END DATE: 05/31/2028

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Modeling transcriptional and post-transcriptional systems for regulating non-genetic heterogeneity in mammalian cells - PROJECT SUMMARY Recent advances of experimental techniques allow quantitative and systems‐wide measurements of non‐ genetic heterogeneity of mammalian cells, which serves as a crucial factor for stem cell dynamics and differentiation potentials, as well as drug resistance of cancer cells. However, it remains challenging to understand many puzzling observations regarding this type of heterogeneity in terms of underlying gene regulatory networks. For example, progenitor cells restore their heterogeneity on timescales of days from subpopulation with extreme gene expression patterns, and these cells switch from plasticity‐enabling dynamics to robust commitment and pattern formation during differentiation; epithelial cells can be distributed in a stable continuum in the epithelial‐mesenchymal spectrum upon receiving signals in tumors. Existing theories provide very limited insights into these important dynamics and patterns. We propose to combine mathematical modeling, new methods of analyzing gene regulatory networks and gene expression data, and experiments in a motor neuron differentiation system, and systems involving epithelial‐mesenchymal transition to study transcriptional and post‐transcriptional mechanisms underlying non‐genetic heterogeneity in mammalian cells. We propose to test a novel theoretical framework of a diverging oscillator for controlling progenitor cell dynamics with both computational methods and experimental systems. We will rigorously formulate and test a new hypothesis of an oscillator‐to‐switch transition for motor neuron differentiation in the developing spinal cord. Stemming from our recent results on surprising post‐transcriptional mechanisms for multistability and oscillation, we will use novel algebraic approaches to establish the relationship between the number of microRNA bindings sites and biologically plausible cell states. We will test the roles of microRNA binding in epithelial cell plasticity experimentally. We will examine the roles of epithelial plasticity and microRNAs in dynamics of lung cancer cells. The success of the proposed study will provide a new theoretical basis and new methods for interpreting and understanding dynamical gene expression data on non‐genetic heterogeneity in mammalian cells. The theories and methods can used as a foundation to develop therapeutics for diseases related to development, tissue degeneration and cancer.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $417,126 )
2025	2025	UNIVERSITY OF TEXAS AT DALLAS	800 WEST CAMPBELL RD.	RICHARDSON	TX	75080	DALLAS	USA	Biomedical Research and Research Training	004	4	9/18/2025	NON-COMPETING CONTINUATION	$390,000
2025	2024	UNIVERSITY OF TENNESSEE	201 ANDY HOLT TOWER	KNOXVILLE	TN	37996	KNOX	USA	Biomedical Research and Research Training	001	2	9/16/2025	NON-COMPETING CONTINUATION	-$362,872
2025	2024	UNIVERSITY OF TENNESSEE	201 ANDY HOLT TOWER	KNOXVILLE	TN	37996	KNOX	USA	Biomedical Research and Research Training	000	2	11/5/2024	NON-COMPETING CONTINUATION	$0
2025	2024	UNIVERSITY OF TEXAS AT DALLAS	800 WEST CAMPBELL RD.	RICHARDSON	TX	75080	DALLAS	USA	Biomedical Research and Research Training	002	3	9/16/2025	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$389,998
2025	2024	UNIVERSITY OF TEXAS AT DALLAS	800 WEST CAMPBELL RD.	RICHARDSON	TX	75080	DALLAS	USA	Biomedical Research and Research Training	003	3	9/17/2025	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
														Subtotal = $417,126

Issue Date FY: 2024 ( Subtotal = $362,873 )
2024	2024	UNIVERSITY OF TENNESSEE	201 ANDY HOLT TOWER	KNOXVILLE	TN	37996	KNOX	USA	Biomedical Research and Research Training	000	2	5/10/2024	NON-COMPETING CONTINUATION	$362,873
														Subtotal = $362,873

Issue Date FY: 2023 ( Subtotal = $330,453 )
2023	2023	UNIVERSITY OF TENNESSEE	201 ANDY HOLT TOWER	KNOXVILLE	TN	37996	KNOX	USA	Biomedical Research and Research Training	000	1	5/25/2023	NEW	$330,453
														Subtotal = $330,453

Grand Total All Awards = $1,110,452

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Modeling transcriptional and post-transcriptional systems for regulating non-genetic heterogeneity in mammalian cells

Award Number: R35GM149531

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 06/01/2023

PERIOD OF PERFORMANCE END DATE: 05/31/2028

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