Wednesday, March 12, 2025
3/12/2025
Protein methylation on arginine and lysine residues represents a type of versatile posttranslational modifications occurring in all eukaryotic organisms. Protein methyltransferases regulate a plethora of cellular processes ranged from gene transcription, RNA splicing, translation, metabolic pathways, to signal transduction. Many protein methyltransferases are found to be overexpressed or mutated in common human diseases such as cancer, inflammation, diabetes, neurological disorders, and infection. Hence, protein methyltransferases are highly promising novel molecular targets in drug discovery. However, biological functions of the majority of protein methyltransferase enzymes in dictating normal physiology and disease pathways are only poorly defined. Our long-term research goal is to elucidate biological pathways whereby key protein methyltransferases contribute to the pathogenesis of recalcitrant diseases such as cancer and infection, and meanwhile to discover new structural chemotypes for protein methyltransferase-targeted therapy. The present research program is aimed at investigating molecular mechanisms and functions of protein methylation catalyzed by key methyltransferases. Our effort is coupled with and aided by development and application of innovative chemical biology methods and tools. Built upon our recent preliminary results, we will implement experiments to elucidate novel molecular mechanisms and regulation of protein arginine methyltransferase (PRMT) activities. We will extend our efforts to investigate the activity, structure and function of untapped protein methyltransferases, including those in different organisms such as infectious pathogens. Particular efforts will be directed to develop isoform-selective modulators and probes for important PRMT members and apply them to elucidate PRMT- regulated cellular pathways and disease processes. Further efforts will be invested to interrogate potential cross- interactions of protein methylation with lysine acetylation in orchestrating biological regulation. The results of the proposed research together will yield an in-depth understanding of the regulatory mechanism and biological significance of protein methylation in the control of normal physiology and disease pathology, and translate laboratory research leads into therapeutic candidates for the treatment of protein methyltransferase-controlled ailments.
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