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Elucidating spatial and temporal dynamics of macrophage polarization using bioluminescence microscopy

Award Number: R35GM142957
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2021
PERIOD OF PERFORMANCE END DATE: 08/31/2026

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Elucidating spatial and temporal dynamics of macrophage polarization using bioluminescence microscopy - Project Summary/Abstract: Macrophage polarization is involved in the progression of normal physiological and pathological processes. The near universal importance of macrophage has led to widescale assessment of the genomic and proteomic to classify their function. However, it is still a challenge to measure macrophage polarization in real time such as in the context of assessing the status of a biomaterial implant or the efficacy of a nanoparticle drug delivery system in a solid tumor. Our preliminary data show that bioluminescence microscopy is able to deliver the protein expression data valuable for macrophage polarization combined the cell tracking capability of traditional imaging system. This research program will address the current gap in real-time assessment of macrophage polarization by 1) further developing the technology to measure macrophage polarization dynamics using bioluminescence microscopy and 2) assessing the intrinsic and extracellular environmental stimuli that are most important for sensing macrophage polarization in a diseased environment context. To accomplish these goals, we will employ ex-vivo culture systems and live bioluminescence imaging. This work will identify novel strategies for measuring macrophage polarization and validate the importance of analyzing polarization in a disease context. Ultimately, this research will lead to new strategies for synthetic biology development, enhancement of diagnostic testing and development of new therapeutic targets. In addition, the knowledge and tools developed can be applied to other cells where polarization is paramount such as stem cell differentiation, chemoattraction, and cell metabolism.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2024	CARNEGIE MELLON UNIVERSITY	5000 FORBES AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Biomedical Research and Research Training	000	4	1/15/2026	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2025 ( Subtotal = $407,788 )
2025	2025	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	002	6	9/23/2025	NON-COMPETING CONTINUATION	$380,741
2025	2024	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	001	5	9/19/2025	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$380,740
2025	2024	CARNEGIE MELLON UNIVERSITY	5000 FORBES AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Biomedical Research and Research Training	000	4	9/18/2025	NON-COMPETING CONTINUATION	-$353,693
														Subtotal = $407,788

Issue Date FY: 2024 ( Subtotal = $353,694 )
2024	2024	CARNEGIE MELLON UNIVERSITY	5000 FORBES AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Biomedical Research and Research Training	000	4	9/12/2024	NON-COMPETING CONTINUATION	$353,694
														Subtotal = $353,694

Issue Date FY: 2023 ( Subtotal = $353,694 )
2023	2023	CARNEGIE MELLON UNIVERSITY	5000 FORBES AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Biomedical Research and Research Training	000	3	8/17/2023	NON-COMPETING CONTINUATION	$353,694
														Subtotal = $353,694

Issue Date FY: 2022 ( Subtotal = $353,694 )
2022	2022	CARNEGIE MELLON UNIVERSITY	5000 FORBES AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Biomedical Research and Research Training	000	2	9/20/2022	NON-COMPETING CONTINUATION	$353,694
														Subtotal = $353,694

Issue Date FY: 2021 ( Subtotal = $343,397 )
2021	2021	CARNEGIE MELLON UNIVERSITY	5000 FORBES AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Biomedical Research and Research Training	000	1	8/30/2021	NEW	$343,397
														Subtotal = $343,397

Grand Total All Awards = $1,812,267

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Elucidating spatial and temporal dynamics of macrophage polarization using bioluminescence microscopy

Award Number: R35GM142957

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2021

PERIOD OF PERFORMANCE END DATE: 08/31/2026

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