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Probing How Living Bacterial Membranes Control Small Molecule Uptake

Award Number: R35GM142928
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/15/2021
PERIOD OF PERFORMANCE END DATE: 06/30/2026

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Probing How Living Bacterial Membranes Control Small Molecule Uptake - Project Summary/Abstract Our research program aims to directly probe how the complexity of living bacterial membranes impacts the adsorption, transport, and domain association of small molecules, including antibiotics. To address these points, we will leverage nonlinear spectroscopy and microscopy techniques, speciﬁcally second harmonic generation, to map the dynamic behavior. A key to our methodology is the ability to conduct the proposed experiments on living cells instead of model systems. For the next 5 years, our program goals are to (1) extract the key factors that inﬂuence the adsorption and membrane organization of small molecule membrane probes, (2) quantitatively assess the adsorption of tetracycline antibiotics and manipulate their movement within and through the membranes of different species of bacteria, and (3) examine the spatial dependence of small molecule-membrane interactions on individual bacteria as well as within bioﬁlms. Together these studies will elucidate the role of how parameters including curvature, membrane domains, and the cell wall mediate small molecule uptake. We envision that this insight will provide new directions in the continued pursuit of improved antibiotics.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $357,073 )
2025	2025	UNIVERSITY OF TENNESSEE	201 ANDY HOLT TOWER	KNOXVILLE	TN	37996	KNOX	USA	Biomedical Research and Research Training	000	5	6/27/2025	NON-COMPETING CONTINUATION	$357,073
														Subtotal = $357,073

Issue Date FY: 2024 ( Subtotal = $357,571 )
2024	2024	UNIVERSITY OF TENNESSEE	201 ANDY HOLT TOWER	KNOXVILLE	TN	37996	KNOX	USA	Biomedical Research and Research Training	000	4	6/19/2024	NON-COMPETING CONTINUATION	$357,571
														Subtotal = $357,571

Issue Date FY: 2023 ( Subtotal = $608,061 )
2023	2023	UNIVERSITY OF TENNESSEE	201 ANDY HOLT TOWER	KNOXVILLE	TN	37996	KNOX	USA	Biomedical Research and Research Training	000	3	8/7/2023	NON-COMPETING CONTINUATION	$358,061
2023	2023	UNIVERSITY OF TENNESSEE	201 ANDY HOLT TOWER	KNOXVILLE	TN	37996	KNOX	USA	Biomedical Research and Research Training	001	3	9/1/2023	SUPPLEMENT FOR EXPANSION	$250,000
														Subtotal = $608,061

Issue Date FY: 2022 ( Subtotal = $358,540 )
2022	2022	UNIVERSITY OF TENNESSEE	1331 CIR PARK DR	KNOXVILLE	TN	37916	KNOX	USA	Biomedical Research and Research Training	000	2	6/17/2022	NON-COMPETING CONTINUATION	$358,540
														Subtotal = $358,540

Issue Date FY: 2021 ( Subtotal = $359,010 )
2021	2021	UNIVERSITY OF TENNESSEE	1331 CIR PARK DR	KNOXVILLE	TN	37916	KNOX	USA	Biomedical Research and Research Training	000	1	9/14/2021	NEW	$359,010
														Subtotal = $359,010

Grand Total All Awards = $2,040,255

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Probing How Living Bacterial Membranes Control Small Molecule Uptake

Award Number: R35GM142928

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/15/2021

PERIOD OF PERFORMANCE END DATE: 06/30/2026

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