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5D subcellular imaging for cell biology

Award Number: R35GM138039
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/15/2020
PERIOD OF PERFORMANCE END DATE: 02/28/2031

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5D subcellular imaging for cell biology - PROJECT SUMMARY Fluorescence microscopy has transformed biological research, enabling super-resolution imaging of biomolecular complexes and advancements in spatial transcriptomics and proteomics. These methods have provided critical insights into the localization and interactions of DNA, RNA and proteins, while deep learning has enhanced the analysis of large microscopy datasets. However, most of these achievements are based on fixed samples, overlooking the dynamic behavior of biomolecules and organelles in live cells. Live-cell imaging faces significant challenges, including photobleaching, phototoxicity, and technical limitations such as high cost, low throughput, and insufficient detection sensitivity. To overcome these barriers, this research proposes the development of innovative 5D subcellular imaging tools that integrate advanced optics, microscopy, photophysics, and deep learning-based analysis. The objective is to enable live-cell imaging with high spatial and temporal resolution, extended observation times, and minimal photodamage. These advancements are designed to transform live-cell imaging, facilitating the study of subcellular structures and biochemical processes in their native states. The proposed imaging approach will prioritize accessibility, efficiency, and impactful applications across the biological sciences.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $361,337 )
2026	2026	THE UNIVERSITY OF CENTRAL FLORIDA BOARD OF TRUSTEES	4000 CENTRAL FLORIDA BLVD	ORLANDO	FL	32816	ORANGE	USA	Biomedical Research and Research Training	001	6	3/12/2026	COMPETING CONTINUATION	$361,337
2026	2024	THE UNIVERSITY OF CENTRAL FLORIDA BOARD OF TRUSTEES	4000 CENTRAL FLORIDA BLVD	ORLANDO	FL	32816	ORANGE	USA	Biomedical Research and Research Training	000	5	3/11/2026	NON-COMPETING CONTINUATION	$0
														Subtotal = $361,337

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2024	THE UNIVERSITY OF CENTRAL FLORIDA BOARD OF TRUSTEES	4000 CENTRAL FLORIDA BLVD	ORLANDO	FL	32816	ORANGE	USA	Biomedical Research and Research Training	000	5	8/12/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $363,408 )
2024	2024	THE UNIVERSITY OF CENTRAL FLORIDA BOARD OF TRUSTEES	4000 CENTRAL FLORIDA BLVD	ORLANDO	FL	32816	ORANGE	USA	Biomedical Research and Research Training	000	5	8/30/2024	NON-COMPETING CONTINUATION	$363,408
														Subtotal = $363,408

Issue Date FY: 2023 ( Subtotal = $359,096 )
2023	2023	THE UNIVERSITY OF CENTRAL FLORIDA BOARD OF TRUSTEES	4000 CENTRAL FLORIDA BLVD	ORLANDO	FL	32816	ORANGE	USA	Biomedical Research and Research Training	000	4	8/25/2023	NON-COMPETING CONTINUATION	$359,096
														Subtotal = $359,096

Issue Date FY: 2022 ( Subtotal = $345,768 )
2022	2022	UNIVERSITY OF CENTRAL FLORIDA BOARD OF TRUSTEES, THE	4000 CENTRAL FLORIDA BLVD	ORLANDO	FL	32816	ORANGE	USA	Biomedical Research and Research Training	000	3	8/24/2022	NON-COMPETING CONTINUATION	$345,768
														Subtotal = $345,768

Issue Date FY: 2021 ( Subtotal = $538,947 )
2021	2021	UNIVERSITY OF CENTRAL FLORIDA BOARD OF TRUSTEES, THE	4000 CENTRAL FLORIDA BLVD	ORLANDO	FL	32816	ORANGE	USA	Biomedical Research and Research Training	000	2	8/17/2021	NON-COMPETING CONTINUATION	$338,947
2021	2021	UNIVERSITY OF CENTRAL FLORIDA BOARD OF TRUSTEES, THE	4000 CENTRAL FLORIDA BLVD	ORLANDO	FL	32816	ORANGE	USA	Biomedical Research and Research Training	001	2	8/23/2021	SUPPLEMENT FOR EXPANSION	$200,000
														Subtotal = $538,947

Issue Date FY: 2020 ( Subtotal = $267,626 )
2020	2020	UNIVERSITY OF CENTRAL FLORIDA BOARD OF TRUSTEES, THE	4000 CENTRAL FLORIDA BLVD	ORLANDO	FL	32816	ORANGE	USA	Biomedical Research and Research Training	000	1	9/11/2020	NEW	$267,626
														Subtotal = $267,626

Grand Total All Awards = $2,236,182

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5D subcellular imaging for cell biology

Award Number: R35GM138039

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/15/2020

PERIOD OF PERFORMANCE END DATE: 02/28/2031

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