Monday, June 30, 2025 6/30/2025

Advanced molecular engineering approach for understanding extracellular vesicle in vivo trafficking mechanism

Award Number: R35GM133794
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/20/2019
PERIOD OF PERFORMANCE END DATE: 01/31/2030

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Advanced molecular engineering approach for understanding extracellular vesicle in vivo trafficking mechanism - ABSTRACT Deciphering extracellular vesicles (EVs) mediated cell-to-cell communications at the molecular precision has been a grand challenge. Mechanistic understanding from current laboratory investigation presents a substantial knowledge gap from clinical translation. Different cell sources derived EVs carry completely different surface receptors and regulatory molecular components. Heterogeneous populations of EVs confound the investigation of key drivers in cellular regulation, for poorly understanding mechanisms on either crossing biological barrier or extracellular matrix accumulation. To date, the molecular mechanisms on tissue targeting specificity are lacking, and the therapeutic potential of different subpopulations of EVs is unknown yet. The proposed research tackles those key challenging questions by introducing a series of advanced molecular engineering approaches for precisely predicting, defining, and in vivo testing EV surface receptor and antigenic presentation. Specifically, project #1 will develop CRISPR imaging technology for understanding molecular mechanism on EV tissue penetration and accumulation. Project #2 will develop artificial intelligent direct-flow generative adversarial model (ExoGAN) enabled construction of specific targeting EVs. Project #3 will develop microfluidic single cell single vesicle transwell system for deciphering effective MHC presenting modulators engaged in EV mediated immunity. This proposal will build a foundation to facilitate mechanistic -driven tissue targeting for advancing precision drug delivery and open broad applications to precision drug delivery.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $381,250 )
2025	2025	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	001	6	1/22/2025	COMPETING CONTINUATION	$381,250
2025	2025	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	005	6	5/23/2025	COMPETING CONTINUATION	$5
2025	2023	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	004	4	5/23/2025	SUPPLEMENT FOR EXPANSION	$0
2025	2023	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	003	5	5/23/2025	SUPPLEMENT FOR EXPANSION	$0
2025	2023	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	000	5	1/15/2025	NON-COMPETING CONTINUATION	$0
2025	2023	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	002	5	5/23/2025	NON-COMPETING CONTINUATION	-$4
														Subtotal = $381,250

Issue Date FY: 2023 ( Subtotal = $411,881 )
2023	2023	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	002	5	8/2/2023	SUPPLEMENT FOR EXPANSION	$23,036
2023	2023	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	001	5	7/31/2023	NON-COMPETING CONTINUATION	$381,250
2023	2023	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	000	4	5/24/2023	SUPPLEMENT FOR EXPANSION	$7,595
														Subtotal = $411,881

Issue Date FY: 2022 ( Subtotal = $496,426 )
2022	2022	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	000	3	11/12/2021	SUPPLEMENT FOR EXPANSION	$46,070
2022	2022	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	001	4	7/25/2022	NON-COMPETING CONTINUATION	$381,250
2022	2022	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	002	4	8/10/2022	SUPPLEMENT FOR EXPANSION	$69,106
														Subtotal = $496,426

Issue Date FY: 2021 ( Subtotal = $516,650 )
2021	2021	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	004	3	8/24/2021	SUPPLEMENT FOR EXPANSION	$135,400
2021	2021	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	003	3	7/27/2021	NON-COMPETING CONTINUATION	$381,250
2021	2020	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	002	2	7/26/2021	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
2021	2020	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	001	2	2/6/2021	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$47,998
2021	2019	UNIVERSITY OF KANSAS CENTER FOR RESEARCH, INC.	2385 IRVING HILL RD	LAWRENCE	KS	66045	DOUGLAS	USA	Biomedical Research and Research Training	000	1	2/6/2021	NEW	-$47,998
														Subtotal = $516,650

Issue Date FY: 2020 ( Subtotal = $381,250 )
2020	2020	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Biomedical Research and Research Training	001	2	9/23/2020	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$381,250
2020	2019	UNIVERSITY OF KANSAS CENTER FOR RESEARCH, INC.	2385 IRVING HILL RD	LAWRENCE	KS	66045	DOUGLAS	USA	Biomedical Research and Research Training	000	1	9/17/2020	NEW	$0
														Subtotal = $381,250

Issue Date FY: 2019 ( Subtotal = $378,750 )
2019	2019	UNIVERSITY OF KANSAS CENTER FOR RESEARCH, INC.	2385 IRVING HILL RD	LAWRENCE	KS	66045	DOUGLAS	USA	Biomedical Research and Research Training	000	1	9/16/2019	NEW	$378,750
														Subtotal = $378,750

Grand Total All Awards = $2,566,207

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Advanced molecular engineering approach for understanding extracellular vesicle in vivo trafficking mechanism

Award Number: R35GM133794

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/20/2019

PERIOD OF PERFORMANCE END DATE: 01/31/2030

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