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Realizing the potential of long-read sequencing technology

Award Number: R35GM133569
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/01/2019
PERIOD OF PERFORMANCE END DATE: 07/31/2029

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Realizing the potential of long-read sequencing technology - Project Summary Long-read sequencing in the form of Paciﬁc Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) have upended the preconceived notion of the capabilities of DNA sequencing applications. However, the molecular biology and computational methods available for long-read sequencing still lag behind the rich ecosystem of methods available for short- read technology in terms of both capability and usability. Because of this, the potential of long-read sequencing methods to beneﬁt biomedical research remains largely unrealized. To address this shortfall, my lab will continue our work on developing and using methods that push the capabilities of long-read sequencing technology. First, we will generate complete isoform-level tissue and cell- type transcriptomes for human and mouse which will be invaluable to the biomedical research community investigating gene and isoform expression using RNA-seq. In addition, access to these transcriptomes will strongly beneﬁt assays that rely on prior knowledge of which isoforms are expressed at what level in any particular cell-type or tissue. Second, we will develop an easy-to-use, ultra-accurate, and read-length agnostic sequencing method which will democratize the use of high-throughput sequencing technology and thereby increase the diversity of the genomics workforce by enabling a much larger number of less well funded labs to perform high-quality high-throughput DNA sequencing assays.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $432,759 )
2025	2025	UNIVERSITY OF CALIFORNIA SANTA CRUZ	1156 HIGH ST	SANTA CRUZ	CA	95064	SANTA CRUZ	USA	Biomedical Research and Research Training	002	7	7/15/2025	NON-COMPETING CONTINUATION	$432,759
2025	2024	UNIVERSITY OF CALIFORNIA SANTA CRUZ	1156 HIGH ST	SANTA CRUZ	CA	95064	SANTA CRUZ	USA	Biomedical Research and Research Training	000	6	11/6/2024	COMPETING CONTINUATION	$0
2025	2023	UNIVERSITY OF CALIFORNIA SANTA CRUZ	1156 HIGH ST	SANTA CRUZ	CA	95064	SANTA CRUZ	USA	Biomedical Research and Research Training	001	5	3/25/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $432,759

Issue Date FY: 2024 ( Subtotal = $443,585 )
2024	2024	UNIVERSITY OF CALIFORNIA SANTA CRUZ	1156 HIGH ST	SANTA CRUZ	CA	95064	SANTA CRUZ	USA	Biomedical Research and Research Training	000	6	6/14/2024	COMPETING CONTINUATION	$443,585
														Subtotal = $443,585

Issue Date FY: 2023 ( Subtotal = $376,904 )
2023	2023	UNIVERSITY OF CALIFORNIA SANTA CRUZ	1156 HIGH ST	SANTA CRUZ	CA	95064	SANTA CRUZ	USA	Biomedical Research and Research Training	000	5	7/26/2023	NON-COMPETING CONTINUATION	$376,904
														Subtotal = $376,904

Issue Date FY: 2022 ( Subtotal = $376,904 )
2022	2022	UNIVERSITY OF CALIFORNIA SANTA CRUZ	1156 HIGH ST	SANTA CRUZ	CA	95064	SANTA CRUZ	USA	Biomedical Research and Research Training	000	4	8/8/2022	NON-COMPETING CONTINUATION	$376,904
														Subtotal = $376,904

Issue Date FY: 2021 ( Subtotal = $376,904 )
2021	2021	UNIVERSITY OF CALIFORNIA, SANTA CRUZ	1156 HIGH ST	SANTA CRUZ	CA	95064	SANTA CRUZ	USA	Biomedical Research and Research Training	000	3	8/9/2021	NON-COMPETING CONTINUATION	$376,904
														Subtotal = $376,904

Issue Date FY: 2020 ( Subtotal = $376,904 )
2020	2020	UNIVERSITY OF CALIFORNIA, SANTA CRUZ	1156 HIGH ST	SANTA CRUZ	CA	95064	SANTA CRUZ	USA	Biomedical Research and Research Training	000	2	7/24/2020	NON-COMPETING CONTINUATION	$376,904
														Subtotal = $376,904

Issue Date FY: 2019 ( Subtotal = $376,904 )
2019	2019	UNIVERSITY OF CALIFORNIA, SANTA CRUZ	1156 HIGH ST	SANTA CRUZ	CA	95064	SANTA CRUZ	USA	Biomedical Research and Research Training	000	1	7/31/2019	NEW	$376,904
														Subtotal = $376,904

Grand Total All Awards = $2,760,864

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Realizing the potential of long-read sequencing technology

Award Number: R35GM133569

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/01/2019

PERIOD OF PERFORMANCE END DATE: 07/31/2029

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