Thursday, November 21, 2024 11/21/2024

Deciphering the role of chemical signals in inflammation with open microfluidic functional assays - U3 Supplement

Award Number: R35GM128648
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Project Abstract Small molecule and protein signals provide a rich vocabulary for cellular communication. The production and consequences of these signals are exquisitely sensitive to cellular context and microenvironment. Dissecting the molecular dialogue between cell types is challenging, and new methods are required to address fundamental questions: What is the downstream biological function of each signaling molecule? How is the biological function different when molecules are present in mixtures or when different cell types are present in the microenvironment? How do microbes—like the bacteria and fungi present in our bodies—affect the molecular landscape? Our lab is developing new tools to probe these questions including (1) microscale co- and multiculture methods that enable precise positioning of cell types to study cell signaling, (2) specialized culture platforms for complex human-bacteria-fungal multikingdom culture, (3) integration of microbial co- and multiculture systems with volatile organic compound (VOC) sampling to study how volatiles mediate microbial dialogue, and (4) at-home biofluid sampling and stabilization platforms to probe the human immune response over time. The parent R35 award has three stated goals: 1) Develop novel microscale co- and multi-culture platforms to study soluble factor signaling and use these tools to elucidate paracrine signaling mechanisms. 2) Develop and validate new readouts for inflammation (e.g., fibrosis, vasodilation) and apply these methods to identify key effector molecules and signaling pathways in inflammation. 3) Develop new analytical methods to stabilize, isolate, and study inflammatory signals. Under the parent R35 award Goal 3, we developed a novel platform that enables at-home blood sampling and RNA stabilization, homeRNA. The homeRNA kit contains a commercially available at-home blood collection device and a custom ‘stabilizer tube’ that our lab engineered to contain a stabilizing solution (e.g., RNAlater to stabilize RNA in blood). Importantly, homeRNA enables longitudinal studies within an individual to capture temporal changes in gene expression signatures resulting from exposures, disease flares, or in response to treatment. homeRNA enables evaluation of mechanistic hypotheses in human populations, providing a complement to our lab’s in vitro microfluidic platforms, which use cell lines or primary cells. In this supplement, my lab will build on our prior U3 Research Supplement (awarded in 2022, prior to renewal of the parent R35 grant), where we established a cohort of U3 women (n=39) who used homeRNA to sample themselves at home during an active COVID-19 infection. We will conduct surveys and interviews to better understand their experiences in the study, ways that future studies can be improved to facilitate inclusion of diverse populations, and ways that remote/telemedicine can increase access to healthcare for U3 women. We will also analyze the samples collected to evaluate RNA integrity and yield for future transcriptomics research.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2024	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	000	7	11/6/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $934,701 )
2024	2024	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	003	7	8/22/2024	SUPPLEMENT FOR EXPANSION	$216,736
2024	2024	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	001	7	7/11/2024	NON-COMPETING CONTINUATION	$419,850
2024	2024	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	002	7	7/17/2024	SUPPLEMENT FOR EXPANSION	$48,115
2024	2024	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	000	6	5/10/2024	SUPPLEMENT FOR EXPANSION	$250,000
														Subtotal = $934,701

Issue Date FY: 2023 ( Subtotal = $419,850 )
2023	2023	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	000	6	7/19/2023	COMPETING CONTINUATION	$419,850
														Subtotal = $419,850

Issue Date FY: 2022 ( Subtotal = $619,110 )
2022	2022	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	001	5	7/18/2022	NON-COMPETING CONTINUATION	$387,700
2022	2022	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	000	4	5/31/2022	SUPPLEMENT FOR EXPANSION	$13,710
2022	2022	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	003	5	9/8/2022	SUPPLEMENT FOR EXPANSION	$0
2022	2022	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	002	5	8/19/2022	SUPPLEMENT FOR EXPANSION	$217,700
														Subtotal = $619,110

Issue Date FY: 2021 ( Subtotal = $475,583 )
2021	2021	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	001	4	7/23/2021	NON-COMPETING CONTINUATION	$392,136
2021	2021	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	000	3	5/27/2021	SUPPLEMENT FOR EXPANSION	$8,871
2021	2021	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	002	4	8/19/2021	SUPPLEMENT FOR EXPANSION	$74,576
														Subtotal = $475,583

Issue Date FY: 2020 ( Subtotal = $527,529 )
2020	2020	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	000	2	5/25/2020	SUPPLEMENT FOR EXPANSION	$69,942
2020	2020	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	001	2	6/3/2020	SUPPLEMENT FOR EXPANSION	$13,101
2020	2020	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	002	3	7/17/2020	NON-COMPETING CONTINUATION	$444,486
														Subtotal = $527,529

Issue Date FY: 2019 ( Subtotal = $478,459 )
2019	2019	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	002	2	7/30/2019	NON-COMPETING CONTINUATION	$459,529
2019	2019	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	001	1	6/19/2019	SUPPLEMENT FOR EXPANSION	$0
2019	2019	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	000	1	5/22/2019	SUPPLEMENT FOR EXPANSION	$18,930
														Subtotal = $478,459

Issue Date FY: 2018 ( Subtotal = $380,605 )
2018	2018	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Biomedical Research and Research Training	000	1	7/27/2018	NEW	$380,605
														Subtotal = $380,605

Grand Total All Awards = $3,835,837

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Deciphering the role of chemical signals in inflammation with open microfluidic functional assays - U3 Supplement

Award Number: R35GM128648

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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