Thursday, May 2, 2024
5/2/2024
PROJECT SUMMARY Mechanisms underlying the origin and maintenance of adult form, and naturally occurring variation in adult form, remain poorly understood. This research program seeks to elucidate how gene activities are translated through cellular behaviors into speci¿c morphological outcomes at adult stages. Such information will con- tribute to understanding patterning and morphogenetic mechanisms essential for postembryonic development, with relevance to human genetic disease, birth defects, aging and regenerative medicine. For these efforts, the work uses pigmentation of zebra¿sh, its close relatives in the genus Danio, and more distantly related teleost ¿shes. Pigment cells in these animals and other vertebrates arise from embryonic neural crest cells that also contribute to a wide variety of other tissues and organs, including most of the peripheral nervous system and craniofacial skeleton. Defects in neural crest derived lineages generally, and pigment cells speci¿cally, are as- sociated with numerous hereditary pathologies as well as cancers, including melanoma. During normal devel- opment, pigment cells that arise either directly from neural crest cells or indirectly through postembryonic stem cell intermediates organize into highly stereotyped, largely two dimensional patterns in the transparent skin. Cell behaviors during pattern formation are readily observed as phenotypes develop, and genetic mechanisms are accessible through mutational analyses and other approaches, both in striped zebra¿sh and in other species having very different adult patterns. The work described here builds on prior effort in this program, and takes an unusually integrative approach to understand pattern and pattern variation, combining manipulative experiments, genetic analysis, high resolution imaging, cutting edge genomics, comparative biology and be- havioral assays. Goals in the coming years are to elucidate: (i) mechanisms by which pigment cell progenitors are speci¿ed for different pigment cell types during development, and how diversi¿cation of cell types has been achieved evolutionarily; (ii) genetic and cellular mechanisms underlying self-organizing interactions among pigment cells that are essential for pattern formation, and how these interactions and permissive factors have changed to generate alternative pattern states among species; (iii) the roles of positional information in the tis- sue environment in setting the location of discrete pattern elements that are essential for establishing pattern, and how such information contributes to qualitatively different types of pattern across species. These efforts will provide novel insights into pattern development and cell type diversi¿cation over both developmental and evolutionary time. General principles uncovered will likely be applicable to a wide range of traits that depend to varying degrees cell type diversi¿cation, self-organizing cellular interactions, and positional information derived from tissue environments. 1
HHS’ Tracking Accountability in Government Grants System (TAGGS) website provides access to detailed descriptions of grants, loans, aggregated direct payments and other types of financial assistance awarded by the HHS Operating Divisions (OPDIVs) and the Office of the Secretary Staff Divisions (STAFFDIVs).
