Wednesday, January 15, 2025
1/15/2025
PARENT AWARD SUMMARY RNA silencing is a gene regulatory mechanism by which small RNAs (18-30 nucleotides) and their Argonaute protein co-factors modulate the expression of both foreign and endogenous mRNAs. Small RNAs play a critical role in maintaining proper gene expression by identifying fully or partially complementary mRNAs and targeting them for either transcriptional or post-transcriptional regulation. The role of RNA silencing in gene regulatory pathways is conserved across most eukaryotes and is thus of fundamental importance to the developmental and cellular biology of humans. C. elegans is an excellent system to study RNA silencing because of its short generation time, its transparency which is ideal for microscopy, and the powerful genetic tools available for genome manipulation. In C. elegans germ cells, proteins involved in RNA silencing are organized into sub- compartments of perinuclear condensates, with each sub-compartment playing a unique and critical role. Many protein factors have been identified that localize to these structures, yet we know little about how they are organized and assembled. In the first part of this proposal, we will identify factors and conditions that promote assembly of RNA silencing components into perinuclear condensates. We will further identify the RNAs in each sub-compartment, with the ultimate goal of dissecting the trajectory of a targeted mRNA through each of its phases: beginning with transcription, continuing through this assemblage of perinuclear condensates, and ultimately with translational repression or degradation by the RNA silencing pathway. Because there are ~27 C. elegans Argonaute proteins, many of which colocalize at P granules, we will next identify the factors contributing to sorting of small RNA into the appropriate Argonaute proteins. This sorting is critical because many Argonaute proteins bind different small RNAs from one another, target distinct groups of mRNAs, and can have very different regulatory effects on these target mRNAs. Finally, we will focus on how protein modifications such as phosphorylation and methylation can regulate RNA silencing pathways, including affecting localization, interacting partners, and dynamics of Argonaute proteins and other key factors. Together, the proposed experiments will uncover the key details of how RNA silencing pathways are organized, how the specificity of the pathways is defined, and what mechanisms regulate these pathways. This work will lead to a better understanding of how small RNAs modulate gene expression in healthy cells, which can ultimately be applied to discerning how changes in these pathways cause misregulation of genes in humans and transition to a disease state.
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