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A validated mouse model of brown fat targeting

Award Number: R21OD034470
ORGANIZATION: OFFICE OF THE DIRECTOR, NIH
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/01/2024
PERIOD OF PERFORMANCE END DATE: 10/22/2025

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A validated mouse model of brown fat targeting - Abstract/Project Summary Mice and human physiology are deeply affected by brown fat through its control of metabolic homeostasis and production of signaling molecules. As such, brown fat specific genetic modifications in mice have arisen as a truly relevant tool to understand brown fat role in controlling physiology with applications in an array of organ systems. For this, Cre-recombinase drivers under control of the UCP1 promoter have been used. In this proposal, we show that the most commonly used mouse model of brown fat targeting is the source of some previously unnoticed negative effects. Thus, our preliminary data supports an unmet critical need to generate a new mouse model to target mature brown adipocytes in an efficient and safe manner. For these reasons, the main goals of this application are to generate (Aim 1), validate (Aim 2) and make widely available (Aim 3) a new model of brown fat targeting. For this, we will use an innovative but validated CRISPR/Cas9 targeting technique to insert Cre-recombinase driven by a defined ucp1 promoter into a safe harbor location. Next, we will use Cre-mediated lineage tracing techniques to address its cellular specificity. Due to the broad interest in brown fat biology, this mouse model is anticipated to be broadly used by scientists in an array of fields of interest to multiple NIH institutes. This new mouse model of brown fat targeting is expected to have a positive impact as a step forward towards developing better preclinical mouse models. We will make our new model available to the community through a mouse repository. For these reasons, this proposal is directed to the FOA PAR-21-167, “Development of Animal Models and Related Materials for Research”.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2025	CHILDRENS HOSPITAL MEDICAL CENTER	3333 BURNET AVE	CINCINNATI	OH	45229	HAMILTON	USA	Research Infrastructure Programs	000	2	11/21/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2025 ( Subtotal = $200,625 )
2025	2025	CHILDRENS HOSPITAL MEDICAL CENTER	3333 BURNET AVE	CINCINNATI	OH	45229	HAMILTON	USA	Research Infrastructure Programs	000	2	7/23/2025	NON-COMPETING CONTINUATION	$200,625
														Subtotal = $200,625

Issue Date FY: 2024 ( Subtotal = $240,750 )
2024	2024	CHILDRENS HOSPITAL MEDICAL CENTER	3333 BURNET AVE	CINCINNATI	OH	45229	HAMILTON	USA	Research Infrastructure Programs	000	1	6/19/2024	NEW	$240,750
														Subtotal = $240,750

Grand Total All Awards = $441,375

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A validated mouse model of brown fat targeting

Award Number: R21OD034470

ORGANIZATION: OFFICE OF THE DIRECTOR, NIH

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/01/2024

PERIOD OF PERFORMANCE END DATE: 10/22/2025

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