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RNA-based tools for developmental and regenerative biology

Award Number: R21GM143565
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2021
PERIOD OF PERFORMANCE END DATE: 08/31/2024

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RNA-based tools for developmental and regenerative biology - Project Summary RNA-based technologies have enabled perturbation and observation of gene function in multicellular organisms, resulting in major discoveries in biomedical research. However, gaining mechanistic insights into developmental and regenerative processes requires the manipulation of gene functions with spatial and temporal precision, a task that remains a major challenge in vertebrate animal models. The overall goal of this exploratory technology development proposal is to develop an innovative and widely applicable molecular toolset, which will enable control of gene expression with spatial and temporal precision. Specifically, we will introduce a new class of versatile RNA-based genetic conditional switches to regulate translation within the developing zebrafish. In our first aim, we will develop RNA switches that are controlled through non-invasive administration of non-toxic small molecules. These chemically-induced switches enable dynamic, titratable, and conditional control of a target gene. We will establish a rapid mammalian- based prototyping platform to prioritize ideal switch candidates for development in the zebrafish model. In our second aim, we will establish a conceptually new optogenetic switch that can be turned on and off using blue light. These novel tools will be developed in zebrafish and benchmarked against existing optogenetic tools including light-activated transcriptional tools. The successful execution of this project will provide a streamlined pipeline to develop new RNA switches that respond to a diversity of orthogonal non-invasive inputs. Given the species-independent machinery, the toolkit of RNA switches developed are likely to be broadly applicable to animal models. By specifically focusing the development of the switches in zebrafish, we will we will contribute a new approach to reprogram and interrogate developmental and regenerative biology.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = -$3,514 )
2025	2022	EAST CAROLINA UNIVERSITY	1000 E 5TH ST	GREENVILLE	NC	27858	PITT	USA	Biomedical Research and Research Training	001	3	10/22/2024	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$1
2025	2022	UNIVERSITY OF WYOMING	1000 E UNIVERSITY AVE	LARAMIE	WY	82071	ALBANY	USA	Biomedical Research and Research Training	000	2	10/22/2024	NON-COMPETING CONTINUATION	-$1
2025	2022	EAST CAROLINA UNIVERSITY	1000 E 5TH ST	GREENVILLE	NC	27858	PITT	USA	Biomedical Research and Research Training	002	3	12/18/2024	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	-$3,514
														Subtotal = -$3,514

Issue Date FY: 2023 ( Subtotal = $4,176 )
2023	2022	EAST CAROLINA UNIVERSITY	1000 E 5TH ST	GREENVILLE	NC	27858	PITT	USA	Biomedical Research and Research Training	001	3	7/13/2023	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$97,011
2023	2022	UNIVERSITY OF WYOMING	1000 E UNIVERSITY AVE DEPARTMENT 3434	LARAMIE	WY	82071	ALBANY	USA	Biomedical Research and Research Training	000	2	7/12/2023	NON-COMPETING CONTINUATION	-$92,835
														Subtotal = $4,176

Issue Date FY: 2022 ( Subtotal = $159,944 )
2022	2022	UNIVERSITY OF WYOMING	1000 E UNIVERSITY AVE DEPARTMENT 3434	LARAMIE	WY	82071	ALBANY	USA	Biomedical Research and Research Training	000	2	8/15/2022	NON-COMPETING CONTINUATION	$159,944
														Subtotal = $159,944

Issue Date FY: 2021 ( Subtotal = $192,140 )
2021	2021	UNIVERSITY OF WYOMING	1000 E UNIVERSITY AVE DEPARTMENT 3434	LARAMIE	WY	82071	ALBANY	USA	Biomedical Research and Research Training	000	1	8/24/2021	NEW	$192,140
														Subtotal = $192,140

Grand Total All Awards = $352,746

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RNA-based tools for developmental and regenerative biology

Award Number: R21GM143565

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2021

PERIOD OF PERFORMANCE END DATE: 08/31/2024

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