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Inverted Spectroscopic Infrared Microscope (ISIM) for High Throughput Multi-Dimensional Cell Assays

Award Number: R21GM138947
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Abstract The goal of this research is to develop a novel spectroscopic cellular assay that will enable us to measure the real-time intra-cellular response of a cell to various extra-cellular stimuli. The uniqueness of our approach relies on several innovations. We will construct an Inverted Spectral Infrared Microscope (ISIM) based on Fourier- transform infrared (FTIR) spectroscopy and combining it with a novel biosensor based on plasmonic nanostructures (metasurfaces). The biosensor will be integrated with multi-well plates to enable high- throughput. The proposed assay will detect biochemical and morphological changes of the cell, with the emphasis on the reorganization of the cellular membrane and its cytoskeleton. The multi-dimensional nature of the spectroscopic data will enable the application of machine learning techniques and improve its sensitivity in comparison with traditional one-dimensional real-time assays. To our knowledge, this will be the first real-time cellular assay that satisfies all of the four requirements below: (i) high throughput, (ii) multi-dimensionality of the collected time-dependent data, (iii) specific focus on biochemical changes of the cell, and (iv) focus on the changes occurring in close proximity of the cellular membrane. The assay will be validated using very common external stimuli of the cell, such as small-molecule compounds acting on G-protein coupled receptors.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = $205,591 )
2022	2022	CORNELL UNIVERSITY	341 PINE TREE RD	ITHACA	NY	14850	TOMPKINS	USA	Biomedical Research and Research Training	000	2	5/30/2022	NON-COMPETING CONTINUATION	$205,591
														Subtotal = $205,591

Issue Date FY: 2021 ( Subtotal = $233,752 )
2021	2021	CORNELL UNIVERSITY	341 PINE TREE RD	ITHACA	NY	14850	TOMPKINS	USA	Biomedical Research and Research Training	000	1	5/2/2021	NEW	$233,752
														Subtotal = $233,752

Grand Total All Awards = $439,343

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Inverted Spectroscopic Infrared Microscope (ISIM) for High Throughput Multi-Dimensional Cell Assays

Award Number: R21GM138947

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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