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A NOVEL CHEMOPROTEOMIC TECHNOLOGY TO REVEAL GLOBAL SMALL MOLECULE-PROTEOME-WIDE INTERACTIONS IN LIVE CELLS.

Award Number: R21GM126264
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Project Summary/Abstract Small molecules are abundant in nature, where they perform various roles in living cells, as enzyme cofactors, signaling molecules in biological networks or as metabolic components. As a consequence of physical interaction with proteins, they may illicit profound changes in protein function that can have physiological outcomes. Several biophysical and biochemical approaches, including high-throughput screening strategies, have been developed in an effort to globally or individually detect protein-metabolite interactions in vitro, in situ or in vivo. The use of affinity tags and protein overexpression has enabled the detection of metabolome-wide small molecule ligands of each protein target in turn. However, these methods are compounded by a necessity to isolate the protein target of interest from the cell, thus providing lesser physiological relevance and complicating downstream validation and analysis. Excluding the use of protein or small molecule arrays, the remaining in situ or in vivo approaches are limited to permit the identification of proteome-wide targets of each small molecule in turn. These technological restrictions translate to a bottleneck in data acquisition and difficulties in validation when determining important functional roles of small molecules in the context of biological networks. Therefore, currently, there are no technologies to globally identify the proteome-wide targets of all small molecules in live cells without isolation and disturbance of the intact targets within the intracellular milieu. We aim to overcome this limitation by developing a new chemoproteomic technology that will permit direct isolation and characterization by mass spectrometry of metabolome-wide small molecules with their respective proteome-wide targets in situ in a single experiment. This truly global approach will deliver a powerful new tool for the investigator of protein-small molecule interactions by enabling the acquisition of the complete protein-metabolite interactome in live cells. We expect that the information gathered by adopting this technology will yield novel insights in small molecule-protein regulation, providing more comprehensive and larger biological networks for the analysis of signaling pathways of physiological relevance, and facilitating comparisons of entire organism protein-small molecule interactions under different experimental conditions in a similar manner to other current –omics disciplines.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = $0 )
2022	2020	PELIAN BIOSCIENCES, LLC	428 OAKMEAD PKWY	SUNNYVALE	CA	94085	SANTA CLARA	USA	Biomedical Research and Research Training	000	3	5/18/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2020 ( Subtotal = $120,258 )
2020	2020	PELIAN BIOSCIENCES, LLC	428 OAKMEAD PKWY	SUNNYVALE	CA	94085	SANTA CLARA	USA	Biomedical Research and Research Training	001	3	2/24/2020	NON-COMPETING CONTINUATION	$20,732
2020	2020	PELIAN BIOSCIENCES, LLC	428 OAKMEAD PKWY	SUNNYVALE	CA	94085	SANTA CLARA	USA	Biomedical Research and Research Training	000	3	2/5/2020	NON-COMPETING CONTINUATION	$186,570
2020	2020	PELIAN BIOSCIENCES, LLC	428 OAKMEAD PKWY	SUNNYVALE	CA	94085	SANTA CLARA	USA	Biomedical Research and Research Training	002	3	6/18/2020	NON-COMPETING CONTINUATION	-$29,577
2020	2019	PELIAN BIOSCIENCES, LLC	428 OAKMEAD PKWY	SUNNYVALE	CA	94085	SANTA CLARA	USA	Biomedical Research and Research Training	003	2	6/18/2020	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	-$57,467
														Subtotal = $120,258

Issue Date FY: 2019 ( Subtotal = $248,959 )
2019	2019	PELIAN BIOSCIENCES, LLC	428 OAKMEAD PKWY	SUNNYVALE	CA	94085	SANTA CLARA	USA	Biomedical Research and Research Training	003	2	6/20/2019	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$98,984
2019	2019	MOLECULAR MEDICINE RESEARCH INSTITUTE	428 OAKMEAD PKWY	SUNNYVALE	CA	94085	SANTA CLARA	USA	Biomedical Research and Research Training	000	1	1/31/2019	NEW	$292,500
2019	2019	PELIAN BIOSCIENCES, LLC	428 OAKMEAD PKWY	SUNNYVALE	CA	94085	SANTA CLARA	USA	Biomedical Research and Research Training	002	2	6/12/2019	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$149,975
2019	2019	MOLECULAR MEDICINE RESEARCH INSTITUTE	428 OAKMEAD PKWY	SUNNYVALE	CA	94085	SANTA CLARA	USA	Biomedical Research and Research Training	001	1	6/12/2019	NEW	-$292,500
														Subtotal = $248,959

Grand Total All Awards = $369,217

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A NOVEL CHEMOPROTEOMIC TECHNOLOGY TO REVEAL GLOBAL SMALL MOLECULE-PROTEOME-WIDE INTERACTIONS IN LIVE CELLS.

Award Number: R21GM126264

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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