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Concurrent volumetric imaging with multimodal optical systems

Award Number: R21EB034938
ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/01/2023
PERIOD OF PERFORMANCE END DATE: 12/31/2025

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Concurrent volumetric imaging with multimodal optical systems - PROJECT SUMMARY The rapid adoption of genetically modified animals and fluorescently labeled biocompatible nanoparticles for drug delivery in biomedical science have increased demand for imaging technologies capable of fast volumetric imaging to enable longitudinal investigations of cell dynamics in their natural environment. The complexity of information required to understand tissue response to injury and treatment has also generated increased interest in multimodal imaging systems that combine complementary performance strengths. In this regard, we have developed an integrated dual-modality imaging system that combines optical coherence microscopy (OCM) and dual-channel confocal fluorescence microscopy (CFM) to enable the simultaneous measurements of fluorescence and reflectance from deep tissue layers. The combined system provides a unique opportunity to inform cells’ behavior in their natural environment by offering complementary information not available with either system alone. CFM interogates the distribution of fluorescently labeled molecules in cells. OCM is a label-free, episcopic method providing information about the cell boundaries, extracellular matrix, and thickness changes in layers of normal and pathogenic tissues. Despite its potential, the integration of these technologies is incomplete without the capability of concurrent volumetric imaging allowing the tracking of cell dynamics progressively in the same specimen. As for scanning- based systems, fast volumetric imaging in OCM and CFM requires dynamic focusing at the level of individual scanning points, which is challenging due to the limited temporal resolution of dynamic focusing devices. This project aims to establish the capability of the tunable acoustic gradient lens, the fastest dynamic focusing technology to date, in OCM and CFM to enable a fast volumetric and concurrent imaging of depth-resolved reflectance and fluorescence from deep tissue layers in vivo. This integration will have a tremendous impact on biomedical research as OCM and CFM technologies remain the most affordable, flexible, and the latter is readily available in many research facilities. We propose the following two specific aims: Aim 1: Enable fast volumetric imaging in confocal fluorescence microscopy. Aim 2: Enable extended depth-of-field in optical coherence microscopy. Significance The ability to concurrently acquire volumetric information such as reflectance and fluorescence rapidly from deep tissue layers will provide a powerful tool for longitudinal investigations into developmental and pathophysiological mechanisms in various research fields and facilitate in vivo cell tracking in the same specimen while increasing the rigor and reproducibility of studies by decreasing the inter-subject variability that can affect the outcomes of cellular processes.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2024	TRUSTEES OF INDIANA UNIVERSITY	107 S INDIANA AVE	BLOOMINGTON	IN	47405	MONROE	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	2	12/5/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $310,278 )
2024	2024	TRUSTEES OF INDIANA UNIVERSITY	107 S INDIANA AVE	BLOOMINGTON	IN	47405	MONROE	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	2	6/28/2024	NON-COMPETING CONTINUATION	$310,278
														Subtotal = $310,278

Issue Date FY: 2023 ( Subtotal = $284,264 )
2023	2023	TRUSTEES OF INDIANA UNIVERSITY	107 S INDIANA AVE	BLOOMINGTON	IN	47405	MONROE	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	1	6/29/2023	NEW	$284,264
														Subtotal = $284,264

Grand Total All Awards = $594,542

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Concurrent volumetric imaging with multimodal optical systems

Award Number: R21EB034938

ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/01/2023

PERIOD OF PERFORMANCE END DATE: 12/31/2025

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