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Hyperspectral chemical imaging via sum-frequency generation microscopic holography

Award Number: R21EB034418
ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/01/2024
PERIOD OF PERFORMANCE END DATE: 04/30/2027

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Hyperspectral chemical imaging via sum-frequency generation microscopic holography - Project Summary: This proposal outlines a new investigator grant (NIBIB Trailblazer R21) for the PI to develop a new holographic platform that enables high-speed, noninvasive 3D chemical imaging with broad biomedical applications, including analysis of tumor microenvironment and real-time imaging of live cell cultures/animal models/engineered tissues. While nonlinear optical microscopy is an attractive approach because it is label-free, nondestructive, and offers higher resolution and richer information than linear microscopy, it usually involves 3D scanning that limits the imaging refresh rate. To obviate this bottleneck, recent research has sought to combine nonlinear optical microscopy with digital holography to vastly improve imaging speed. However, current implementations either lack chemical selectivity or require tuning the laser wavelength in order to measure vibrational spectra. To achieve a deeper understanding of biomedical processes at the molecular level, there is clearly a significant need to further improve imaging speed and obtain rich spectral information. The long-term goal is to develop a new nonlinear digital microscopic holography approach capable of high-speed 3D imaging with spectroscopic vibrational contrast: i.e., 5D imaging in spatial, temporal, and spectral dimensions, in live cell cultures and animal tissues. This transformational tool will enable discoveries of disease mechanisms and new treatment paradigms. This application’s objective is to demonstrate the feasibility of a new approach to achieving time-domain hyperspectral microscopic holography through vibrationally resonant (VR) sum-frequency generation (SFG) and third-order sum-frequency generation (TSFG). Using mid-infrared photons to resonantly excite vibrational modes will enable chemical mapping of different functional groups in specimens, while the nonlinear processes offer submicron resolution. Combining SFG and TSFG in one instrument will enable multimodal probing of non-centrosymmetric sample components as well as other components. Hyperspectral holography will enable 3D imaging and simultaneous recovery of the signal field’s amplitude, phase, and spectral frequency in a single time scan. Three specific aims will be pursued: Aim 1 is to demonstrate hyperspectral VR-SFG microscopic holography and validate its performance. Based on comparison to the phase-sensitive multiplex VR-SFG microscopy previously demonstrated in the applicant’s hands, a single holographic interferogram can be measured with a 4-μs exposure time and a signal-to-noise ratio of 10, with further improvement in signal-to-noise ratio expected. Aim 2 is to demonstrate multimodal VR- SFG/TSFG and to accelerate the acquisition rate by ~10x via compressive sensing. Aim 3 is to expand the spectral range to the fingerprint region through building a new mid-infrared light source. The approach is innovative because it integrates concepts in a new unproven format for imaging with unprecedented speed and vibrational spectroscopic contrast. The proposed research is significant because it is expected to vertically advance nonlinear digital microscopic holography for chemical mapping with submicron/subcellular resolutions.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $133,245 )
2026	2026	UNIVERSITY OF CALIFORNIA IRVINE	160 ALDRICH HALL	IRVINE	CA	92697	ORANGE	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	3	5/11/2026	NON-COMPETING CONTINUATION	$133,245
														Subtotal = $133,245

Issue Date FY: 2025 ( Subtotal = $192,024 )
2025	2025	UNIVERSITY OF CALIFORNIA IRVINE	160 ALDRICH HALL	IRVINE	CA	92697	ORANGE	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	2	4/14/2025	NON-COMPETING CONTINUATION	$192,024
														Subtotal = $192,024

Issue Date FY: 2024 ( Subtotal = $224,104 )
2024	2024	UNIVERSITY OF CALIFORNIA IRVINE	160 ALDRICH HALL	IRVINE	CA	92697	ORANGE	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	1	7/23/2024	NEW	$224,104
														Subtotal = $224,104

Grand Total All Awards = $549,373

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Hyperspectral chemical imaging via sum-frequency generation microscopic holography

Award Number: R21EB034418

ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/01/2024

PERIOD OF PERFORMANCE END DATE: 04/30/2027

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