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Identification of diffusible small molecules that regulate replication of Porphyromonas gingivalis

Award Number: R21DE034093
ORGANIZATION: NATIONAL INSTITUTE OF DENTAL AND CRANIOFACIAL RESEARCH
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 04/01/2025
PERIOD OF PERFORMANCE END DATE: 03/31/2027

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Identification of diffusible small molecules that regulate replication of Porphyromonas gingivalis - Project Summary Porphyromonas gingivalis (Pg) is a late-colonizer with a demonstrated role in the etiology of periodontitis and implicated as a risk factor in several systemic conditions. Our previous work showed that population size, which determines the availability of a still unidentified endogenous diffusible small molecule(s), limits the in vitro growth and the in vivo colonization and virulence of Pg. Our work also showed that the inability of Pg to grow in vitro and colonize in vivo from a small inoculum was overcome by forming a partnership with the ubiquitous Gram- negative early colonizer Veillonella parvula (Vp). In preliminary studies we show that intermediates of menaquinone biosynthesis may play a role stimulating growth of low cell-density Pg and may be produced by certain strains of Pg and by Vp. However, other strains of Pg capable of supporting their own growth via small diffusible cues present in their spent media may utilize a different strategy as they do not have the machinery for menaquinone biosynthesis. Accordingly, the overall goal of this application is to establish the identity of the growth-promoting cues produced by different strains of Pg and by Vp that support Pg replication from a low cell density population. Therefore, in this proposal we will utilize chemical and molecular genetic strategies to identify the soluble cues produced by Pg 381, Pg W83 and Vp that stimulate growth of Pg from low cell density inocula. We anticipate this work will elucidate the mechanisms regulating cell density-dependent replication in different strains of Pg and the manner in which inter-species interactions with early biofilm colonizers, such as Vp, can help Pg overcome the growth barrier and allow its replication and establishment in the oral cavity.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $201,250 )
2026	2026	THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK	520 LEE ENTRANCE STE 211	AMHERST	NY	14228	ERIE	USA	Oral Diseases and Disorders Research	000	2	3/24/2026	NON-COMPETING CONTINUATION	$201,250
														Subtotal = $201,250

Issue Date FY: 2025 ( Subtotal = $241,500 )
2025	2025	THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK	520 LEE ENTRANCE STE 211	AMHERST	NY	14228	ERIE	USA	Oral Diseases and Disorders Research	000	1	3/26/2025	NEW	$241,500
														Subtotal = $241,500

Grand Total All Awards = $442,750

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Identification of diffusible small molecules that regulate replication of Porphyromonas gingivalis

Award Number: R21DE034093

ORGANIZATION: NATIONAL INSTITUTE OF DENTAL AND CRANIOFACIAL RESEARCH

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 04/01/2025

PERIOD OF PERFORMANCE END DATE: 03/31/2027

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