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Cellular Identity of the Intact HIV Reservoir

Award Number: R21AI189303
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 06/11/2025
PERIOD OF PERFORMANCE END DATE: 05/31/2027

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Cellular Identity of the Intact HIV Reservoir - ABSTRACT Persistence of the HIV reservoir is a major barrier to cure. Many cure-directed clinical trials use novel interventions to reactivate the reservoir for immune targeting and reservoir reduction. Improving the targeting and elimination of HIV reservoir requires a cell-by-cell understanding of where reservoir is and how reservoir persists after cure interventions. However, current strategies to identify the multi-modal biology of cells with integrated provirus are not able to fully resolve the intact proviral reservoir: they cannot detect provirus in closed chromatin and cannot discern intact from defective provirus. This proposal will work to interrogate cellular programs of the total intact reservoir using two new sequencing approaches to address the problems of proviral intactness and integration in closed chromatin. In aim 1, we will test the hypothesis that CD4+ T cells with provirus in closed chromatin are transcriptionally distinct from cells with provirus in open chromatin. In aim 2, we will test the hypothesis that cells with intact provirus have distinct transcriptional, epigenetic, and clonal features. We will therefore work to identify the distinct features of the intact reservoir and to generate a technical and analytic pipeline for adjunctive use across multiple cure strategies.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $489,500 )
2025	2025	THE CHILDREN'S HOSPITAL OF PHILADELPHIA	3401 CIVIC CENTER BLVD	PHILADELPHIA	PA	19104	PHILADELPHIA	USA	Allergy and Infectious Diseases Research	000	1	6/11/2025	NEW	$489,500
														Subtotal = $489,500

Grand Total All Awards = $489,500

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Cellular Identity of the Intact HIV Reservoir

Award Number: R21AI189303

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 06/11/2025

PERIOD OF PERFORMANCE END DATE: 05/31/2027

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