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Regulation of biofilm formation and pathogenesis in bordetella pertussis

Award Number: R21AI123805
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 04/20/2016
PERIOD OF PERFORMANCE END DATE: 03/31/2020

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Regulation of biofilm formation and pathogenesis in bordetella pertussis - DESCRIPTION (provided by applicant): The Gram-negative bacterium Bordetella pertussis is a human restricted pathogen that causes severe respiratory infection and the disease known as whooping cough or pertussis. The recent rise in B. pertussis infections and the disease in the USA and other high income countries, along with waning immunity to current commercial pertussis vaccines necessitate research on understanding the pathogenic mechanisms employed by this bacterium. Our overall hypothesis is that the formation of biofilms is essential for persistent colonization of the respiratory tract and pathogenesis of B. pertussis. Control of biofilm development in the largely animal pathogen B. bronchiseptica is mediated by BpsR, a highly conserved transcriptional regulator. Additionally, BpsR dually activates or represses the expression of a large number of genes which have roles in virulence, transcriptional regulation, metabolic and other cellular processes. Nothing is known about the role of BpsR in B. pertussis. Functional studies of BpsR in B. pertussis have been hindered by the inability to construct a mutant strain lacking the bpsR gene. This suggests that bpsR is an essential gene in B. pertussis. We hypothesize that by regulating the expression of genes involved in essential metabolic pathways, pathogenesis and biofilm formation, BpsR controls the ability of B. pertussis to survive in the respiratory tract. In Specific Aim 1, we will utilize a tetracycline dependent conditional silencing system to examine its role in laboratory growth, gene expression and biofilm development. In Specific Aim 2, these studies will be extended to an intranasal mouse model of B. pertussis infection and biofilm development to identify its role in colonization of and formation of biofilms on respiratory organs.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2020 ( Subtotal = $0 )
2020	2017	OHIO STATE UNIVERSITY, THE	1960 KENNY RD	COLUMBUS	OH	43210	FRANKLIN	USA	Microbiology and Infectious Diseases Research	000	3	9/28/2020	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
														Subtotal = $0

Issue Date FY: 2019 ( Subtotal = $0 )
2019	2017	OHIO STATE UNIVERSITY, THE	1960 KENNY RD	COLUMBUS	OH	43210	FRANKLIN	USA	Microbiology and Infectious Diseases Research	000	3	1/31/2019	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
														Subtotal = $0

Issue Date FY: 2018 ( Subtotal = $0 )
2018	2017	OHIO STATE UNIVERSITY, THE	1960 KENNY RD	COLUMBUS	OH	43210	FRANKLIN	USA	Microbiology and Infectious Diseases Research	001	3	11/21/2017	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$232,500
2018	2017	WAKE FOREST UNIVERSITY HEALTH SCIENCES	MEDICAL CENTER BLVD	WINSTON SALEM	NC	27157	FORSYTH	USA	Microbiology and Infectious Diseases Research	000	2	11/20/2017	NON-COMPETING CONTINUATION	-$232,500
2018	2017	OHIO STATE UNIVERSITY, THE	1960 KENNY RD	COLUMBUS	OH	43210	FRANKLIN	USA	Microbiology and Infectious Diseases Research	003	3	12/28/2017	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$57,016
2018	2016	WAKE FOREST UNIVERSITY HEALTH SCIENCES	MEDICAL CENTER BLVD	WINSTON SALEM	NC	27157	FORSYTH	USA	Microbiology and Infectious Diseases Research	002	1	12/28/2017	NEW	-$57,016
														Subtotal = $0

Issue Date FY: 2017 ( Subtotal = $232,500 )
2017	2017	WAKE FOREST UNIVERSITY HEALTH SCIENCES	MEDICAL CENTER BLVD	WINSTON SALEM	NC	27157	FORSYTH	USA	Microbiology and Infectious Diseases Research	000	2	3/15/2017	NON-COMPETING CONTINUATION	$232,500
														Subtotal = $232,500

Issue Date FY: 2016 ( Subtotal = $193,750 )
2016	2016	WAKE FOREST UNIVERSITY HEALTH SCIENCES	MEDICAL CENTER BOULEVARD	WINSTON SALEM	NC	27157	FORSYTH	USA	Microbiology and Infectious Diseases Research	000	1	4/19/2016	NEW	$193,750
														Subtotal = $193,750

Grand Total All Awards = $426,250

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Regulation of biofilm formation and pathogenesis in bordetella pertussis

Award Number: R21AI123805

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 04/20/2016

PERIOD OF PERFORMANCE END DATE: 03/31/2020

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