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In Vitro Reconstitution of Centralspindlin-Mediated Transport

Award Number: R16GM159670
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2025
PERIOD OF PERFORMANCE END DATE: 05/31/2029

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In Vitro Reconstitution of Centralspindlin-Mediated Transport - Project summary/abstract This proposal aims to address the knowledge gap in our understanding of the molecular mechanisms through which centralspindlin regulates cytokinesis. Cytokinesis is the last act of cell division, in which cleavage furrow ingression produces physically distinct daughter cells. Errors in cytokinesis can lead to cancer. During cytokinesis, centralspindlin, a motor protein complex, is crucial for the localization of key furrowing components and other proteins to the site of cleavage furrow formation. Although previous in vivo studies have highlighted centralspindlin’s essential role, the exact mechanisms remain unclear. We propose to clarify these through the in vitro reconstitution of centralspindlin-mediated transport. The overall objective of this project is to define the molecular mechanisms through which centralspindlin transports required cargos along microtubules to direct furrow formation. Our lab has established conditions to stably express full-length centralspindlin and developed methods for the in vitro reconstitution of centralspindlin-mediated transport. Our long-term goal is to reconstitute cytokinesis using purified centralspindlin and other regulatory components. Our central hypotheses is that centralspindlin-mediated transport along microtubules is a regulated mechanism to localize the components that direct furrow formation and cytoplasmic abscission. We will test our hypothesis by identifying the features of centralspindlin required for robust microtubule plus-end localization, determining the target cargos transported by centralspindlin, and exploring the factors that regulate centralspindlin-mediated transport. This study is innovative because it employs novel methods to address previously unresolved questions about centralspindlin. The project is significant as it will provide a precise understanding of cytokinesis and offer new potential targets for cancer treatments. Additionally, this project will help establish a research community of underrepresented minority students through my laboratory at the University of Hawaii at Hilo, the most ethnically diverse campus among national universities.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $145,500 )
2025	2025	UNIVERSITY OF HAWAII	200 W KAWILI ST	HILO	HI	96720	HAWAII	USA	Biomedical Research and Research Training	000	1	8/27/2025	NEW	$145,500
														Subtotal = $145,500

Grand Total All Awards = $145,500

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In Vitro Reconstitution of Centralspindlin-Mediated Transport

Award Number: R16GM159670

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2025

PERIOD OF PERFORMANCE END DATE: 05/31/2029

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