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Single-cell immune landscape in the oral dysplasia's malignant transformation

Award Number: R16GM150790
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/01/2023
PERIOD OF PERFORMANCE END DATE: 06/30/2027

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Single-cell immune landscape in the oral dysplasia's malignant transformation - About 7.9-27.6% of oral dysplasia, a premalignant lesion, transit to the invasive oral cavity squamous cell carcinoma (OCSCC). Prognostic biomarkers are critically needed to determine patients with oral dysplastic lesions at risk for malignant transformation and to guide the targeted development of novel therapies. Our goal is to establish a single-cell atlas of premalignant immune microenvironment (PRIME) in oral dysplasia and to identify immune features that predict the malignant transformation to OCSCC. We propose an innovative approach that combines high-dimensional imaging mass cytometry (IMC) and machine learning predictive modeling (iEN) to analyze a total of ~200 Formalin-Fixed Paraffin-Embedded (FFPE) patient tongue biopsies from the Oral Pathology Archive at the University of the Pacific (UOP), Arthur A. Dugoni School of Dentistry. IMC is a new multiplex imaging technology which combines high- dimensional mass cytometry with microscopy. Immune Elastic Net (iEN) is a machine learning algorithm specifically developing for the analysis of high-dimensional mass cytometry data. We plan to identify immune features that differentiate oral dysplasia severity (Aim 1) and predict OCSCC malignant transformation (Aim 2). In addition, we will analyze the iEN-selected immune features at UOP Han’s lab by conducting multiplex immunofluorescence (mIF) staining on the whole sections (Aim 1&2) to validate and generalize the IMC findings. The proposed research will establish immune landscape in oral dysplasia and identify biomarkers to predict the malignant transformation. It also provides an opportunity for predental or dental students participating in translational research and collaborating with multidisciplinary team to identify biomarkers to improve oral pathology diagnosis.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $176,255 )
2025	2025	UNIVERSITY OF PACIFIC	3601 PACIFIC AVE	STOCKTON	CA	95211	SAN JOAQUIN	USA	Biomedical Research and Research Training	000	3	5/30/2025	NON-COMPETING CONTINUATION	$176,255
														Subtotal = $176,255

Issue Date FY: 2024 ( Subtotal = $171,880 )
2024	2024	UNIVERSITY OF PACIFIC	3601 PACIFIC AVE	STOCKTON	CA	95211	SAN JOAQUIN	USA	Biomedical Research and Research Training	000	2	7/1/2024	NON-COMPETING CONTINUATION	$171,880
														Subtotal = $171,880

Issue Date FY: 2023 ( Subtotal = $177,898 )
2023	2023	UNIVERSITY OF PACIFIC	3601 PACIFIC AVE	STOCKTON	CA	95211	SAN JOAQUIN	USA	Biomedical Research and Research Training	000	1	7/31/2023	NEW	$177,898
														Subtotal = $177,898

Grand Total All Awards = $526,033

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Single-cell immune landscape in the oral dysplasia's malignant transformation

Award Number: R16GM150790

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/01/2023

PERIOD OF PERFORMANCE END DATE: 06/30/2027

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