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Investigation of the G protein-coupled receptor FSHR-1 in multi-tissue neuromuscular signaling in normal and oxidative stress conditions

Award Number: R15NS112918
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/20/2022
PERIOD OF PERFORMANCE END DATE: 07/31/2025

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Investigation of the G protein-coupled receptor FSHR-1 in multi-tissue neuromuscular signaling in normal and oxidative stress conditions - PROJECT SUMMARY/ABSTRACT Neurological and neurodegenerative disorders characterized by imbalances in synaptic transmission are often linked to oxidative damage. Modification of synaptic signaling in the face of damage is critical for nervous system function. Inter-tissue signaling is key to stress-induced synaptic modulation, but is not fully understood. My long-term goal is to determine the molecular mechanisms controlling synaptic transmission in the presence and absence of oxidative stress. G protein-coupled receptors (GPCRs) are regulators of synaptic transmission and multi-tissue stress responses, yet the details of relevant GPCR pathways are largely unknown. Recent work from my lab and others identified roles for the conserved GPCR FSHR-1 in regulating neuromuscular signaling and oxidative stress responses. FSHR-1 is the sole C. elegans homolog of a family of mammalian glycopeptide (GP) hormone receptors that control gonad development and function; Fshr deficiency is linked to depression and brain oxidative stress in mice. In C. elegans, intestinal FSHR-1 promotes organism survival during infection and oxidative stress; neuronal FSHR-1 can cell non-autonomously regulate intestinal oxidative stress responses. My lab found that fshr-1 null animals have neuromuscular defects exacerbated by oxidative stress, but where and how FSHR-1 regulates synaptic signaling under normal or stress conditions and the mechanisms by which FSHR-1 is activated are unknown. The objective of this proposal is to determine how FSHR-1 controls signaling at the C. elegans neuromuscular junction (NMJ) via activities in multiple cell types in the presence and absence of oxidative stress. My preliminary data indicate FSHR-1 can act in neurons and the intestine to promote muscle excitation. Our data further indicate this effect may be due to FSHR-1’s ability to act cell non-autonomously to promote cholinergic synaptic vesicle release from motor neurons and suggest a candidate GP ligand and downstream signaling pathways FSHR-1 may use exert to its effects at the NMJ. My central hypothesis is that FSHR-1 acts in a subset of non-NMJ cells downstream of the -GP FLR-2 and/or other peptide ligands to activate pathways involving GS and/or the lipid kinase SPHK-1 to indirectly promote acetylcholine release and muscle excitation. Aim 1 will use genome editing, fluorescence localization and calcium imaging, cell-specific protein degradation, and behavior to determine neuronal and non-neuronal sites of FSHR-1 action in controlling NMJ function in normal and oxidative stress conditions. Aim 2 will use genetics, imaging, and behavior to determine if downstream FSHR-1 effectors in other contexts mediate FSHR-1’s effects on NMJ activity. Aim 3 will use complementary genetic epistasis and biochemical approaches to identify FSHR-1 ligands relevant for its NMJ effects. This research is innovative in its use of a whole animal model to explore inter-tissue signaling by a conserved GPCR regulating synaptic transmission in diverse conditions. It is significant in defining novel roles for FSHR-1, which controls diverse processes across phylogeny, and may lay groundwork for new therapies for synaptic dysfunction and oxidative stress, hallmarks of aging and disease.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2022	BUTLER UNIVERSITY	4600 SUNSET AVE	INDIANAPOLIS	IN	46208	MARION	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	2/19/2026	NEW	$0
														Subtotal = $0

Issue Date FY: 2025 ( Subtotal = $516,307 )
2025	2025	BUTLER UNIVERSITY	4600 SUNSET AVE	INDIANAPOLIS	IN	46208	MARION	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	7/31/2025	COMPETING CONTINUATION	$516,307
														Subtotal = $516,307

Issue Date FY: 2023 ( Subtotal = $0 )
2023	2022	BUTLER UNIVERSITY	4600 SUNSET AVE	INDIANAPOLIS	IN	46208	MARION	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	6/8/2023	NEW	$0
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = $422,267 )
2022	2022	BUTLER UNIVERSITY	4600 SUNSET AVE	INDIANAPOLIS	IN	46208	MARION	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	1	9/19/2022	NEW	$0
2022	2022	BUTLER UNIVERSITY	4600 SUNSET AVE	INDIANAPOLIS	IN	46208	MARION	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	8/19/2022	NEW	$422,267
														Subtotal = $422,267

Grand Total All Awards = $938,574

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Investigation of the G protein-coupled receptor FSHR-1 in multi-tissue neuromuscular signaling in normal and oxidative stress conditions

Award Number: R15NS112918

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/20/2022

PERIOD OF PERFORMANCE END DATE: 07/31/2025

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