Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Nuclear Role of the Proteasome in Synaptic Plasticity

Award Number: R15NS098405
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2016
PERIOD OF PERFORMANCE END DATE: 05/31/2021

Group Awards By:

View Award Description

Nuclear Role of the Proteasome in Synaptic Plasticity - Elucidating the mechanisms by which synapses are altered for long-term memory storage is crucial for understanding both normal and abnormal functions of the nervous system. Investigations over the years have established that new gene transcription and translation of the newly transcribed genes is required for maintenance of long-term synaptic plasticity and consolidation of long-term memory. Research during the last two decades has revealed that proteolysis by the ubiquitin-proteasome pathway (UPP) has an essential role in synaptic plasticity and memory. Much of the work on the UPP has been focused on its traditional function, namely, degradation of substrate proteins. It is now becoming clear that the proteasome has other roles in the cell such as regulation of transcription. Studies carried out on non-neuronal cell types have shown that the proteasome binds to promoters of actively transcribed genes and assists in transcription. A part of the proteasome called the 19S regulatory complex contains several ATPases among which Rpt1 has been shown to play a critical role in transcription. We will investigate the role of the proteasome in transcription by focusing on Rpt1. We will use hippocampal late phase long-term potentiation (L-LTP) as a model system for our studies. Our preliminary data show that Rpt1 translocates to the nucleus in hippocampal slices in response to L-LTP- inducing stimuli. Also, our results show that Rpt1 binds to specific promoters of the brain-derived neurotrophic factor (BDNF) gene. Our first aim is to use a high-throughput sequencing method in combination with chromatin immunoprecipitation with Rpt1 antibodies to identify the transcriptional targets of Rpt1. Our second aim is to test the hypothesis that the function of nuclear Rpt1 is critical for transcription and L-LTP maintenance. This project will lay the groundwork for elucidating the unconventional roles of the proteasome in transcription required for long-term synaptic plasticity which will have significant implications for understanding normal long-term memory as well as loss of memory seen in many diseases and disorders of the brain. Furthermore, by providing hands-on research experience to undergraduate students, this project will significantly enhance the training of future biomedical scientists.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2021 ( Subtotal = -$30,622 )
2021	2016	GEORGIA COLLEGE & STATE UNIVERSITY	231 W HANCOCK ST	MILLEDGEVILLE	GA	31061	BALDWIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	9/27/2021	NEW	-$30,622
														Subtotal = -$30,622

Issue Date FY: 2020 ( Subtotal = $0 )
2020	2016	GEORGIA COLLEGE & STATE UNIVERSITY	231 W HANCOCK ST	MILLEDGEVILLE	GA	31061	BALDWIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	9/28/2020	NEW	$0
														Subtotal = $0

Issue Date FY: 2016 ( Subtotal = $381,357 )
2016	2016	GEORGIA COLLEGE & STATE UNIVERSITY	CAMPUS BOX 021	MILLEDGEVILLE	GA	31061	BALDWIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	8/25/2016	NEW	$381,357
														Subtotal = $381,357

Grand Total All Awards = $350,735

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Nuclear Role of the Proteasome in Synaptic Plasticity

Award Number: R15NS098405

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2016

PERIOD OF PERFORMANCE END DATE: 05/31/2021

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer