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Linking transgenerational epigenetic inheritance to gene expression and lifespan in C. elegans

Award Number: R15GM144861
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/21/2021
PERIOD OF PERFORMANCE END DATE: 08/31/2025

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Linking transgenerational epigenetic inheritance to gene expression and lifespan in C. elegans - PROJECT SUMMARY The experience of our ancestors has important consequences for our own health. Studies in humans and animal models have shown that stress or trauma experienced in one generation can affect the cognition, behavior, and physiology of subsequent descendants for multiple generations. However, the mechanisms of transgenerational inheritance have proven challenging to study, partially due to the scarcity of epigenetic phenotypes that can be traced through multiple generations. Thus, we have developed a unique model for transgenerational epigenetic inheritance in the nematode Caenorhabditis elegans that allows us to link molecular changes with phenotypic effects over dozens of generations. Our long-term goal is to leverage this model to understand how chromatin landscapes shape gene expression, cell fate, and physiology. In our cells, genomes are packaged into chromatin, in which DNA is wrapped around cores of histone proteins. Modifications are added and removed from histones, regulating access to DNA and therefore, gene expression. The addition of two methyl groups to lysine 9 of histone 3 (H3K9me2) is associated with gene repression, while addition to lysine 4 of the same histone (H3K4me) is associated with gene activation. We discovered that a mutation in either WDR-5, which belongs to an H3K4 methylating complex, or a mutation in JHDM-1, a putative demethylase for H3K9me2, causes the gradual, genome-wide accumulation of repressive H3K9me2. This accumulation causes a gradual lifespan extension in both mutants: wdr-5 mutants acquire longevity after twenty generations, while jhdm-1 mutants acquire longevity after eight generations. We will use this model of transgenerational epigenetic inheritance to probe the consequences caused by the inappropriate inheritance of repressive H3K9me2. We hypothesize that generational changes in repressive H3K9me2 affect gene expression and organismal physiology to impact health and extend lifespan. This proposal combines unbiased genomic analyses with classical genetic approaches in two Specific Aims: 1. Identify which specific pathways respond to the transgenerational accumulation of repressive H3K9me2 by examining health, gene expression, and chromatin accessibility; and 2. Determine how H3K9me2 accumulation genetically interacts with the known lifespan-extending pathway of lipid metabolism using epistasis analysis. Our approach offers two main advantages: as self-fertilizing hermaphrodites, homozygous populations of C. elegans are genetically identical, allowing us to distinguish epigenetic traits from genetic ones; and importantly, C. elegans can survive perturbations to epigenetic inheritance that cause embryonic lethality in other animals. The mechanisms of epigenetic regulation are highly conserved among all eukaryotes, including nematodes and mammals. Therefore, our proposed research will provide fundamental insight into how the inheritance of chromatin landscapes affects gene expression in human health and disease.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2021	UNIVERSITY OF MASSACHUSETTS LOWELL	220 PAWTUCKET ST STE 400	LOWELL	MA	01854	MIDDLESEX	USA	Biomedical Research and Research Training	000	1	2/17/2026	NEW	$0
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2023	UNIVERSITY OF MASSACHUSETTS LOWELL	600 SUFFOLK ST STE 450	LOWELL	MA	01854	MIDDLESEX	USA	Biomedical Research and Research Training	001	1	6/24/2024	SUPPLEMENT FOR EXPANSION	$0
2024	2021	UNIVERSITY OF MASSACHUSETTS LOWELL	600 SUFFOLK ST STE 450	LOWELL	MA	01854	MIDDLESEX	USA	Biomedical Research and Research Training	000	1	6/24/2024	NEW	$0
														Subtotal = $0

Issue Date FY: 2023 ( Subtotal = $31,926 )
2023	2023	UNIVERSITY OF MASSACHUSETTS	600 SUFFOLK ST STE 212	LOWELL	MA	01854	MIDDLESEX	USA	Biomedical Research and Research Training	000	1	5/26/2023	SUPPLEMENT FOR EXPANSION	$31,926
														Subtotal = $31,926

Issue Date FY: 2021 ( Subtotal = $374,300 )
2021	2021	UNIVERSITY OF MASSACHUSETTS	600 SUFFOLK ST STE 415	LOWELL	MA	01854	MIDDLESEX	USA	Biomedical Research and Research Training	000	1	9/21/2021	NEW	$374,300
														Subtotal = $374,300

Grand Total All Awards = $406,226

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Linking transgenerational epigenetic inheritance to gene expression and lifespan in C. elegans

Award Number: R15GM144861

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/21/2021

PERIOD OF PERFORMANCE END DATE: 08/31/2025

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