Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Selenide-based electrocatalytic sensors for sensitive peroxynitrite detection in biological media: a bottom-up approach for functional interface design

Award Number: R15GM140405
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2021
PERIOD OF PERFORMANCE END DATE: 08/31/2025

Group Awards By:

View Award Description

Selenide-based electrocatalytic sensors for sensitive peroxynitrite detection in biological media: a bottom-up approach for functional interface design - Project Summary: Background and Challenge: Peroxynitrite (OONO-) emerged as a potent cytotoxic compound and has been implicated in a host of pathophysiological conditions. Peroxynitrite is the primary product of the in vivo reaction of nitric oxide and superoxide anion-radical. The multifaceted physiologic reactions of this compound are directly implicated in a number of pathologies including cardiovascular disease, immune response, chronic inflammation, and sepsis, to cite a few. According to recent statistics by the American Heart Association, just cardiovascular disease alone claims about 7 deaths every 4 minutes. On the other hand, sepsis affects 1.7 million adults in the United States each year and potentially contributes to more than 250,000 deaths. Just these two statistics are staggering and make the footprint of this deadly biological analyte an important priority. The common thread that links peroxynitrite to all cited pathologies is its potent reactivity toward most cellular components including DNA, proteins, and lipids in cell membranes. Substantial oxidations and other transformations of proteins, DNA, and lipids contribute to the disruption of key cellular functions. Assessing peroxynitrite’s deleterious effects and examining hypotheses of its potential signaling roles cannot be achieved without first accurately measuring and monitoring its concentration. This task is however inherently difficult due to low submicromolar concentrations under physiologic conditions coupled with its high reactivity. Sensitive and accurate measurement of peroxynitrite is crucial in order to shed light on the illusive pathophysiologic roles of this metabolite. Some of the known detection methods for peroxynitrite include oxidation of fluorescent probes, EPR spectroscopy, chemiluminescence, immunohistochemistry, and probe nitration; however, these are more difficult to apply for real-time quantification due to their inherent complexity. The electrochemical detection of peroxynitrite is a simpler and more convenient technique for application in biological settings. However, a systematic development of the right electrode interface that enhances the sensitivity and selectivity for this molecule is lacking. Recently, several synthetic organic selenides have been prepared as antioxidants in medicinal chemistry. Electrochemical data in our hands showed that some organoselenium compounds have specific redox activity with peroxynitrite in solution. For these reasons, we believe that an electrode interface decorated with organoselenides attached to the surface will potentially serve as catalytic entities for mediated PON electrocatalytic determination. Our proposal: In this work, we propose to develop a functional thin film material based on defined organic selenides chemically attached on graphite electrodes and use this interface in sensitive electrochemical determination of peroxynitrite. This bottom-up interface design approach is innovative because it allows us to design an electrocatalytic interface for the detection and determination of peroxynitrite driven by molecular and electronic properties of the organic selenides used. This is driven by the overall hypothesis that the redox-rich organoselenium compounds will allow us to use them as active redox catalytic centers tethered to the electrode surface to electrocatalytically measure PON in solution. The work will pursue three specific aims including: 1) developing a selenide-decorated electrode for PON determination using a reference compound, followed by 2) generating a library of selenides with varying substituents on the selenium catalytic center and test the catalytic properties of the resulting modified interface towards PON determination; and finally 3) miniaturizing the best performing catalytic interfaces by transferring the process to ultramicroelectrodes (carbon fiber) to prepare a single-body PON microsensor for use under biological settings. Significance: The successfu


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2021	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	000	1	2/17/2026	NEW	$0
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2023	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	001	1	9/10/2024	SUPPLEMENT FOR EXPANSION	$0
2024	2021	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	000	1	9/9/2024	NEW	$0
														Subtotal = $0

Issue Date FY: 2023 ( Subtotal = $100,000 )
2023	2023	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	000	1	6/6/2023	SUPPLEMENT FOR EXPANSION	$100,000
														Subtotal = $100,000

Issue Date FY: 2021 ( Subtotal = $447,128 )
2021	2021	CLEVELAND STATE UNIVERSITY	2121 EUCLID AVE	CLEVELAND	OH	44115	CUYAHOGA	USA	Biomedical Research and Research Training	000	1	8/16/2021	NEW	$447,128
														Subtotal = $447,128

Grand Total All Awards = $547,128

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Selenide-based electrocatalytic sensors for sensitive peroxynitrite detection in biological media: a bottom-up approach for functional interface design

Award Number: R15GM140405

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2021

PERIOD OF PERFORMANCE END DATE: 08/31/2025

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer