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Regulation of phosphatidic acid phosphatase during lipogenesis in the oleaginous yeast Yarrowia lipolytica Phosphatidic acid phosphatase in Yarrowia lipolytica

Award Number: R15GM124602
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Abstract Phosphatidic acid phosphatase (PAP) catalyzes the committed step in the biosynthesis of triacylglycerol (TAG) which is the dephosphorylation of phosphatidic acid (PA) to yield diacylglycerol (DAG). By the nature of its reaction PAP controls the cellular levels of PA and TAG. In Saccharomyces cerevisiae, deletion of the PAH1-encoded PAP enzyme (i.e. Scpah1Δ mutant) causes a 90 % reduction in cellular TAG levels. Also, the accumulation of PA in the Scpah1Δ causes the derepression of the phospholipid biosynthetic genes which results in increased phospholipid amounts and is manifested by the expansion of the nuclear/ER membrane. Deregulation of the human PAP enzymes, lipins, is associated with several diseases, like obesity and lipodystrophy. In this competitive grant application, we will identify the genes that encode for PAP activity in Y. lipolytica and examine their role in lipid metabolism (specific aim 1). Y. lipolytica cells exposed to high glucose amounts enter a lipogenic phase where TAG biosynthesis is induced. This results in the accumulation of TAG in the cells which can become as high as 90% of the total lipids. We will examine the role and regulation of the Y. lipolytica PAP enzymes in this metabolism (specific aim 2).


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2023 ( Subtotal = $0 )
2023	2017	ALABAMA A & M UNIVERSITY	4900 MERIDIAN STREET NW	NORMAL	AL	35762	MADISON	USA	Biomedical Research and Research Training	000	1	10/1/2022	NEW	$0
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = -$341 )
2022	2017	ALABAMA A & M UNIVERSITY	4900 MERIDIAN STREET NW	NORMAL	AL	35762	MADISON	USA	Biomedical Research and Research Training	000	1	3/2/2022	NEW	-$341
														Subtotal = -$341

Issue Date FY: 2021 ( Subtotal = $0 )
2021	2017	ALABAMA A & M UNIVERSITY	4900 MERIDIAN ST N	HUNTSVILLE	AL	35810	MADISON	USA	Biomedical Research and Research Training	000	1	3/2/2021	NEW	$0
														Subtotal = $0

Issue Date FY: 2017 ( Subtotal = $315,215 )
2017	2017	ALABAMA A & M UNIVERSITY	4900 MERIDIAN ST N	HUNTSVILLE	AL	35810	MADISON	USA	Biomedical Research and Research Training	000	1	8/29/2017	NEW	$315,215
														Subtotal = $315,215

Grand Total All Awards = $314,873

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Regulation of phosphatidic acid phosphatase during lipogenesis in the oleaginous yeast Yarrowia lipolytica Phosphatidic acid phosphatase in Yarrowia lipolytica

Award Number: R15GM124602

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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