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Dissecting the cAMP-mediated circuitry of stress-induced sleep in Caenorhabditis elegans

Award Number: R15GM122058
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/20/2016
PERIOD OF PERFORMANCE END DATE: 08/31/2019

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Dissecting the cAMP-mediated circuitry of stress-induced sleep in Caenorhabditis elegans - Project/Summary Vertebrate and invertebrate sleep are controlled by conserved signaling molecules, which suggests that sleep is evolutionarily ancient. Caenorhabditis elegans provides a powerful means for rapid identification and characterization of sleep-regulating pathways with single cell resolution because of their fast generation time and a simple and defined 302-celled nervous system. One pathway shown to regulate sleep in all animals involves cyclic adenosine monophosphate (cAMP), a ubiquitous second messenger that largely functions in neurons. C. elegans sleep in response to cellular stress caused by environmental insults (stress-induced sleep), an intriguing behavioral model for studying sleep during sickness. This project will define where cAMP is functioning in the C. elegans nervous system during stress-induced sleep. It will also determine where and how stress and sleep intersect in the nervous system. In Aim 1, we will identify the neurons in which cAMP is functioning and measure changes in cAMP levels in live animals while they sleep. To accomplish this, we will use a red light activated adenylyl cyclase that allows for cell-specific induction of cAMP in vivo and we will use a biosensor to measure cAMP changes in real-time during sleep. In Aim 2, we will test the hypothesis that the master stress sensor adenosine monophosphate-activated protein kinase (AMPK) is functioning in the same neurons as cAMP, downstream of protein kinase A, and is activated during sleep in response to cellular stress. In Aim 3 we will connect these newly identified sleep neurons to the known circuitry of stress induced sleep and test the hypothesis that AMPK is functioning downstream of PKA.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2020 ( Subtotal = -$2,615 )
2020	2016	ST JOSEPH'S UNIVERSITY	5600 CITY AVE	PHILADELPHIA	PA	19131	PHILADELPHIA	USA	Biomedical Research and Research Training	000	1	2/8/2020	NEW	-$2,615
														Subtotal = -$2,615

Issue Date FY: 2016 ( Subtotal = $324,000 )
2016	2016	ST JOSEPH'S UNIVERSITY	5600 CITY AVENUE	PHILADELPHIA	PA	19131	PHILADELPHIA	USA	Biomedical Research and Research Training	000	1	9/20/2016	NEW	$324,000
														Subtotal = $324,000

Grand Total All Awards = $321,385

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Dissecting the cAMP-mediated circuitry of stress-induced sleep in Caenorhabditis elegans

Award Number: R15GM122058

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/20/2016

PERIOD OF PERFORMANCE END DATE: 08/31/2019

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