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Follow That Cell: Motility Analysis of L-plastin Mutant Zebrafish

Award Number: R15GM120664
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2016
PERIOD OF PERFORMANCE END DATE: 03/25/2022

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Follow That Cell: Motility Analysis of L-plastin Mutant Zebrafish - PROJECT ABSTRACT Motile cells are critical for the immune system, yet aberrant cell motility is the hallmark of tumors. How cells regulate movement is therefore an important issue. In this application, we explore the genetics of cell motility by experimentally manipulating in zebrafish a key component of the cytoskeleton– the actin-bundling protein L-plastin. L-plastin is normally expressed only in white blood cells, but is upregulated in many types of cancer cells and is frequently studied as an unfavorable prognostic marker for this disease. However the fundamental role of L-plastin in these motile cell populations remains unclear. For the study of this protein, the zebrafish provides an optimal combination of genetic and cellular features. Zebrafish have only one copy of the L-plastin gene, facilitating comparison with the corresponding locus in humans and mice. Genetic engineering in zebrafish is now highly efficient, allowing the rapid production of effective knock-out, knock-in and reporter lines. Finally, the transparent tissues of zebrafish allow live visualization of both normal and modified cell movements in almost any organ. Building on our previous work, this project will use zebrafish L-plastin knockout lines to examine the impact of this protein on cell division, cell adhesion, and immune cell migration. These studies will contribute to our understanding of L-plastin function in embryogenesis, immune response and tumor spread-- all contexts where proper cell migration is critical. In addition to evaluating the links between actin bundling, the cytoskeleton, and cell migration, the proposed work will train diverse undergraduates at our institution for future scientific careers.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = -$21,476 )
2024	2020	DEPAUL UNIVERSITY	1 E JACKSON BLVD	CHICAGO	IL	60604	COOK	USA	Biomedical Research and Research Training	000	2	4/16/2024	COMPETING CONTINUATION	-$21,476
														Subtotal = -$21,476

Issue Date FY: 2023 ( Subtotal = $0 )
2023	2020	DEPAUL UNIVERSITY	1 E JACKSON BLVD	CHICAGO	IL	60604	COOK	USA	Biomedical Research and Research Training	001	2	11/9/2022	COMPETING CONTINUATION	$22,359
2023	2020	DEPAUL UNIVERSITY	1 E JACKSON BLVD	CHICAGO	IL	60604	COOK	USA	Biomedical Research and Research Training	000	2	11/1/2022	COMPETING CONTINUATION	-$22,359
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = -$313,621 )
2022	2020	DE PAUL UNIVERSITY	1 E JACKSON BLVD	CHICAGO	IL	60604	COOK	USA	Biomedical Research and Research Training	001	2	4/4/2022	COMPETING CONTINUATION	-$300,000
2022	2016	DEPAUL UNIVERSITY	1 E JACKSON BLVD	CHICAGO	IL	60604	COOK	USA	Biomedical Research and Research Training	000	1	10/1/2021	NEW	-$13,621
														Subtotal = -$313,621

Issue Date FY: 2021 ( Subtotal = $0 )
2021	2020	DE PAUL UNIVERSITY	1 E JACKSON BLVD	CHICAGO	IL	60604	COOK	USA	Biomedical Research and Research Training	001	2	2/2/2021	COMPETING CONTINUATION	$5,770
2021	2016	DE PAUL UNIVERSITY	1 E JACKSON BLVD	CHICAGO	IL	60604	COOK	USA	Biomedical Research and Research Training	000	1	2/2/2021	NEW	-$5,770
														Subtotal = $0

Issue Date FY: 2020 ( Subtotal = $454,896 )
2020	2020	DE PAUL UNIVERSITY	1 E JACKSON BLVD	CHICAGO	IL	60604	COOK	USA	Biomedical Research and Research Training	001	2	7/27/2020	COMPETING CONTINUATION	$454,896
2020	2016	DE PAUL UNIVERSITY	1 E JACKSON BLVD	CHICAGO	IL	60604	COOK	USA	Biomedical Research and Research Training	000	1	7/17/2020	NEW	$0
														Subtotal = $454,896

Issue Date FY: 2016 ( Subtotal = $443,089 )
2016	2016	DEPAUL UNIVERSITY	25 E. JACKSON BLVD.	CHICAGO	IL	60604	COOK	USA	Biomedical Research and Research Training	000	1	8/25/2016	NEW	$443,089
														Subtotal = $443,089

Grand Total All Awards = $562,889

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Follow That Cell: Motility Analysis of L-plastin Mutant Zebrafish

Award Number: R15GM120664

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2016

PERIOD OF PERFORMANCE END DATE: 03/25/2022

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