Thursday, July 3, 2025 7/3/2025

Sympathetic regulation of inflammation from sleep fragmentation

Award Number: R15GM117534
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/21/2016
PERIOD OF PERFORMANCE END DATE: 04/30/2025

Group Awards By:

View Award Description

Sympathetic regulation of inflammation from sleep fragmentation - Project Summary Primary sleep abnormalities (insomnia, shift work, and obstructive sleep apnea) promote an inflammatory environment and are associated with the development of cardiovascular and metabolic disease. However, the mechanisms underlying these relationships are poorly understood. The proposed research examines hormonal mechanisms that underlie the inflammatory response to sleep fragmentation (SF) using pharmacological approaches in mice. Previous R15-funded research in our lab examined the effect of sympathetic nervous system (SNS) activation upon inflammatory responses to sleep loss. In this renewal application, the role that activation of the hypothalamic-pituitary-adrenal (HPA) axis plays in modulating inflammation from acute and chronic SF will be investigated. Aim 1 will examine whether glucocorticoids mediate the onset of SF-induced inflammation and the activation of microglia, an important contributor to neuroinflammation. Specifically, mice will be adrenalectomized, sham-adrenalectomized, or adrenalectomized and rescued with exogenous glucocorticoids and then subjected to acute and chronic SF. Pro- and antiinflammatory gene and protein expression will be assessed using RT-PCR and multiplex Luminex protein arrays, respectively. Immunocytochemistry will be used to assess microglia activation. These findings will be validated through pharmacological inhibition of glucocorticoid production and binding to glucocorticoid receptors. Instead of a classic anti-inflammatory effect, it is predicted that glucocorticoids will prime SF-induced inflammation. Aim 2 will examine the time course of glucocorticoid release and pro-inflammatory responses to acute and chronic SF. Taken together, the proposed research will assess the importance of the adrenocortical response in mediating inflammation during sleep loss, and provide unique training opportunities for undergraduates interested in conducting biomedical research in endocrinology, immunology, and the neurosciences.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = -$30,748 )
2025	2021	WESTERN KENTUCKY UNIVERSITY	1906 COLLEGE HEIGHTS BLVD # 11002	BOWLING GREEN	KY	42101	WARREN	USA	Biomedical Research and Research Training	000	2	6/23/2025	COMPETING CONTINUATION	-$30,748
														Subtotal = -$30,748

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2021	WESTERN KENTUCKY UNIVERSITY	1906 COLLEGE HEIGHTS BLVD # 11002	BOWLING GREEN	KY	42101	WARREN	USA	Biomedical Research and Research Training	000	2	3/12/2024	COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2021 ( Subtotal = $424,360 )
2021	2021	WESTERN KENTUCKY UNIVERSITY	1906 COLLEGE HEIGHTS BLVD #11026	BOWLING GREEN	KY	42101	WARREN	USA	Biomedical Research and Research Training	001	2	3/26/2021	COMPETING CONTINUATION	$429,768
2021	2016	WESTERN KENTUCKY UNIVERSITY	1906 COLLEGE HEIGHTS BLVD #11026	BOWLING GREEN	KY	42101	WARREN	USA	Biomedical Research and Research Training	000	1	1/31/2021	NEW	-$5,408
														Subtotal = $424,360

Issue Date FY: 2016 ( Subtotal = $400,803 )
2016	2016	Western Kentucky University	1906 COLLEGE HEIGHTS BOULEVARD	BOWLING GREEN	KY	42101	WARREN	USA	Biomedical Research and Research Training	000	1	9/21/2016	NEW	$400,803
														Subtotal = $400,803

Grand Total All Awards = $794,415

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Sympathetic regulation of inflammation from sleep fragmentation

Award Number: R15GM117534

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/21/2016

PERIOD OF PERFORMANCE END DATE: 04/30/2025

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer