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APTAMERS TO DISTINGUISH FUNCTIONAL MODIFICATION OF TARGET PROTEINS BY AFFINITY PR

Award Number: R15GM081854
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/01/2007
PERIOD OF PERFORMANCE END DATE: 06/30/2011

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APTAMERS TO DISTINGUISH FUNCTIONAL MODIFICATION OF TARGET PROTEINS BY AFFINITY PR - DESCRIPTION (provided by applicant): The emergence of functional genomics has led to a growing need for analyzing molecular variants and post-translational modifications of proteins that often determine the biological results of changes at the genomic level. The proposed work is aimed at utilizing aptamers, oligonucleotide ligands for specific target molecules, to distinguish between these molecular variants. To accomplish this, affinity probe capillary electrophoresis (APCE) will be applied as a rapid method to detect fluorescently labeled aptamers that are bound to their targets. In particular, the ability of aptamers for vascular endothelial growth factor (VEGF), a protein implicated in tumor growth, to bind selected peptide domains of the VEGF protein will be investigated. After establishing reliable APCE methods for a selected peptide domain, specific modified peptides that incorporate phosphoserine and/or phosphothreonine will be investigated as models of typical post-translational modifications. Capillary electrophoresis aptamer selection will further identify oligonucleotide sequences that preferentially bind specific peptides. These studies will lead to the development of aptamer arrays for individual molecules, each targeting a particular domain or functional modification of the protein. Such panels would be novel molecular tools for rapid and simple monitoring of disease-linked functional changes in protein molecules with potential applications in drug screening and disease diagnosis. This work will lead to the development of aptamers that target specific domains or functional modifications of target proteins. Such aptamers could be used for signaling the presence of proteins associated with particular disease states, or potentially as pharmaceutical agents that can block or modify the action of these proteins.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2011 ( Subtotal = $0 )
2011	2007	SANTA CLARA UNIVERSITY	**	SANTA CLARA	CA	95053	SANTA CLARA	USA	Biomedical Research and Research Training	000	1	12/17/2010	NEW	$0
														Subtotal = $0

Issue Date FY: 2009 ( Subtotal = $38,548 )
2009	2009	SANTA CLARA UNIVERSITY	C/O SPONSORED PROJECTS OFFICE 500 E	SANTA CLARA	CA	95053	SANTA CLARA	USA	Trans-NIH Recovery Act Research Support	000	1	9/30/2009	SUPPLEMENT FOR EXPANSION	$38,548
														Subtotal = $38,548

Issue Date FY: 2007 ( Subtotal = $193,930 )
2007	2007	SANTA CLARA UNIVERSITY	**	SANTA CLARA	CA	95053	SANTA CLARA	USA	Biomedical Research and Research Training	000	1	6/25/2007	NEW	$193,930
														Subtotal = $193,930

Grand Total All Awards = $232,478

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APTAMERS TO DISTINGUISH FUNCTIONAL MODIFICATION OF TARGET PROTEINS BY AFFINITY PR

Award Number: R15GM081854

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/01/2007

PERIOD OF PERFORMANCE END DATE: 06/30/2011

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