Friday, March 14, 2025
3/14/2025
DESCRIPTION (provided by applicant): NOD-like receptors (NLRs) are cytosolic sensors which initiate innate immune responses against various stimulants. Some NLRs are a part of multiprotein complexes known as inflammasomes which lead to the activation of caspase-1 and subsequent production of IL-1¿ and IL-18. We unexpectedly identified that NLRP12 is a crucial mediator of lung inflammation in response to secondhand smoke (SHS). The proposed studies will address the role of NLRP12 in SHS-induced lung inflammation in both mice and humans. NLRP12 (aka NALP12/MONARCH-1/PYPAF-7), was the first NLR which was shown to induce IL-1¿ maturation via the interaction with ASC. Furthermore, a single report regarding the role of NLRP12 in bacterial recognition determined that NLRP12-deficient mice were more susceptible, showed enhanced bacterial burden in the spleen along with attenuated production of IL-18, IL-1¿, and IFN-? after subcutaneous or intraperitoneal Yersinia pestis infection. In addition, a recent study involving human subjects demonstrated a crucial role of IL-1¿ in the pathophysiology of autoinflammatory syndromes also called NLRP12-associated disorders (NLRP12AD). However, the role of NLRP12 in secondhand smoke-induced COPD/emphysema has not been explored. Our preliminary data demonstrate that: 1) NLRP12 is upregulated in the COPD lungs; 2) SHS exposure upregulates NLRP12 expression in human airway epithelial cells and human alveolar macrophages as well as murine lungs; 3) as compared to other NLRs (NOD1, NOD2, NLRP1, NLRP3, and NLRC4), NLRP12 activation leads to much higher production of IL-1¿, IL-18 and IL-17 in murine lungs following SHS exposure; 4) SHS exposure causes reduced cellular recruitment to the lungs of NLRP12 KO mice; and 5) NLRP12 physically interacts with NOD2 (NLR involved in sensing of invasive microbes) and RIP2 (a common adaptor molecule for both TLRs and NLRs) in alveolar macrophages upon exposure to cigarette smoke extract (CSE) The central hypothesis of this proposal is that interaction of SHS with the host leads to NALP12 activation in the lung followed by IL-1¿, IL-18 and IL-17 production which subsequently contributes to inflammation/emphysema. The aims are: 1) to determine NALP12-dependent SHS-induced pulmonary inflammation in mice; 2) to examine whether myeloid and/or resident cell expressed NALP12 contributes to SHS-induced lung inflammation; 3) NALP12-dependent SHS extract-induced inflammatory responses in isolated human alveolar macrophages; and 4) to identify whether NLRP12 activation involves interaction with other cytosolic NLRs, potentially leading to inflammation and emphysema. Unique resources include NALP12 knockout mice and human alveolar macrophages. The R15 mechanism will help to train undergraduates including minority students in the field of emerging and devastating lung diseases such as COPD/Emphysema.
HHS’ Tracking Accountability in Government Grants System (TAGGS) website provides access to detailed descriptions of grants, loans, aggregated direct payments and other types of financial assistance awarded by the HHS Operating Divisions (OPDIVs) and the Office of the Secretary Staff Divisions (STAFFDIVs).