Tuesday, July 1, 2025 7/1/2025

Seed-derived Therapeutic Protein for Acute Ischemic Stroke

Award Number: R03NS095246
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/30/2015
PERIOD OF PERFORMANCE END DATE: 08/31/2017

Group Awards By:

View Award Description

Seed-derived Therapeutic Protein for Acute Ischemic Stroke - Program Director/Principal Investigator (Last, first, middle): Wang, Kevin, Yueju A stroke occurs when a blood clot or a narrowing of blood vessels prevents blood from getting to the brain. Globally, stroke is the second leading cause of death among people over the age of 60 years. It is also a major source of serious, long-term disability. The only clot- busting drug approved for the treatment of acute ischemic stroke (AIS) disease is a recombinant human tissue plasminogen activator (rt-PA) obtained from Chinese Hamster Ovary cell (CHO) lines. However, yields of rt-PA produced in CHO cells are relatively low, while the dose of rt-PA required for treatment is high, making this therapy very expensive for developing countries. Therefore, a more efficient method of providing rt-PA would greatly reduce the cost and beof great benefit to patients suffering from acute ischemic stroke disease. The objective of the proposed project is to develop a low-cost, plant seed-based platform for the large scale production of functional t-PA for treatment of acute ischemic stroke patients. Our preliminary data revealed that overexpression of several blood-clot dissolving serine protease genes in plant leaves resulted in death of the leaf tissue. In contrast, our preliminary data also indicated that a recombinant vampire bat plasminogen activator (DSPA¿1) could be reliably produced in seeds with sufficient activity to dissolve human blood clots. In this project, appropriate plant expression vectors will be developed to target t-PA gene expression and production to seeds in order to obtain high levels of expression and increased stability of the end product. Transgenic rt-PA plant lines will be generated and in vitro evaluation of seed- derived rt-PA, such as fibrin degradatin and human clot-dissolving assay, will be performed. The overall goal is to develop a seed-based platform in plants for potential large scale, low- cost production of functional rt-PA for the treatment of AIS in humans. 1 Research Strategy


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = -$1,525 )
2022	2015	NORTHEASTERN STATE UNIVERSITY	600 N GRAND AVE	TAHLEQUAH	OK	74464	CHEROKEE	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	10/1/2021	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	-$1,525
														Subtotal = -$1,525

Issue Date FY: 2018 ( Subtotal = -$9,447 )
2018	2016	NORTHEASTERN STATE UNIVERSITY	600 N GRAND AVE	TAHLEQUAH	OK	74464	CHEROKEE	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	3	1/24/2018	NON-COMPETING CONTINUATION	-$9,447
2018	2015	NORTHEASTERN STATE UNIVERSITY	600 N GRAND AVE	TAHLEQUAH	OK	74464	CHEROKEE	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	1/24/2018	NEW	$0
														Subtotal = -$9,447

Issue Date FY: 2016 ( Subtotal = $64,527 )
2016	2016	NORTHEASTERN STATE UNIVERSITY	600 N. GRAND AVE.	TAHLEQUAH	OK	74464	CHEROKEE	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	002	3	8/5/2016	NON-COMPETING CONTINUATION	$64,527
2016	2015	NORTHEASTERN OKLAHOMA STATE UNIVERSITY	600 N. Grand Ave	TAHLEQUAH	OK	74464	CHEROKEE	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	6/30/2016	NEW	-$36,680
2016	2015	NORTHEASTERN STATE UNIVERSITY	600 N. GRAND AVE.	TAHLEQUAH	OK	74464	CHEROKEE	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	2	6/30/2016	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$36,680
														Subtotal = $64,527

Issue Date FY: 2015 ( Subtotal = $62,277 )
2015	2015	NORTHEASTERN OKLAHOMA STATE UNIVERSITY	600 N. Grand Ave	TAHLEQUAH	OK	74464	CHEROKEE	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	9/23/2015	NEW	$62,277
														Subtotal = $62,277

Grand Total All Awards = $115,832

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Seed-derived Therapeutic Protein for Acute Ischemic Stroke

Award Number: R03NS095246

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/30/2015

PERIOD OF PERFORMANCE END DATE: 08/31/2017

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer