Friday, May 9, 2025
5/9/2025
Neural algorithms underlying diversity in visual feature integration - PROJECT SUMMARY We here focus on determining the algorithms that enable highly similar visual information to be transformed into diverse, behaviorally relevant outputs. We also seek to determine the mechanisms that generate these algorithms. Understanding how visual information is transformed into representations relevant for behavior is key for restoring sensorimotor transformations in those who are blind or visually impaired, or suffer from sensory processing disorders. For our visual inputs, we use looming stimuli, the 2-D projections of an object approaching on a direct collision course. Looming stimuli elicit a conserved diversity in behavioral responses across species that are necessary for survival. This diversity is thought to emerge through parallel sensorimotor processing pathways that differentially transform visual features of a looming stimulus into motor outputs. Limited access to both visual feature encoding and visual feature integrating circuit components has however limited the development and biological validation of the algorithms utilized across pathways. We circumvent these limitations by using Drosophila melanogaster that provides the necessary electrophysiological and genetic access to the cell types that participate in these sensorimotor transformations. Our preliminary data suggest looming information is transformed within eight descending sensorimotor pathways (DN) that receive features of looming stimuli from up to six optic lobe columnar projection neuron (OLCPN) cell types. In this interdisciplinary grant, we capitalize on the complementary expertise of Dr. von Reyn (PI), who has pioneered electrophysiological, behavioral, and genetic methods for investigating feature integration within OLCPN and DN, and Dr. Ausborn (co-PI), who has broad expertise in the development of mechanistic biophysical circuit models for the analysis of neural computations within mammalian and invertebrate systems. Here we characterize the extent to which different DN intrinsic properties and circuit mechanisms account for the observed output diversity. In Aim 1 we combine electrophysiology, RNAi silencing, and computational modeling to establish, at a molecular level, intrinsic integration mechanisms for each DN. In Aim 2, we combine electrophysiology, optogenetics, and computational modeling to determine OLCPN synaptic inputs to DN. In Aim 3, through concurrent model and experimental probing, we evaluate the dominant mechanisms that determine looming feature integration algorithms utilized across the DN population. This project will provide a thorough understanding of general principles for transforming sensory information into higher order, behaviorally relevant representations.
HHS’ Tracking Accountability in Government Grants System (TAGGS) website provides access to detailed descriptions of grants, loans, aggregated direct payments and other types of financial assistance awarded by the HHS Operating Divisions (OPDIVs) and the Office of the Secretary Staff Divisions (STAFFDIVs).