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Discovery and analysis of the C. elegans neuronal gene expression network (CeNGEN)

Award Number: R01NS100547
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/25/2017
PERIOD OF PERFORMANCE END DATE: 12/31/2027

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Discovery and analysis of the C. elegans neuronal gene expression network (CeNGEN) - PROJECT SUMMARY Decades of research have established that gene expression in the nervous system is highly specific and dynamic. Different types of neurons express dramatically different genes and process RNAs to generate different isoforms. At each developmental stage, neurons express complements of RNA that together drive them along appropriate trajectories toward their ultimate fate. Similarly, in mature neurons, differential gene expression and processing are key determinants of structure, function, and disease susceptibility. However, although these phenomena are well established, a deep and broad analysis—involving genome-wide, nervous system-wide data—is not available for any organism. Ideally one would like to know, across all neuron types and all developmental stages, exactly how the genome is transcribed and processed into functional RNAs. Such detailed and complete knowledge of differential gene expression within the nervous system would help elucidate the logic and cellular mechanisms that generate neuronal diversity. Further, by comparing dynamic differential gene expression across different sexes in all neuron types, the genetic basis of neuronal sexual dimorphism can be elucidated. The C. elegans Neuronal Gene Expression Map & Network (CeNGEN) project is a multi-lab consortium with the goal of understanding gene expression in the C. elegans nervous system. Here, using a variety of RNAseq approaches, we propose to understand gene expression as neurons differentiate and acquire sexual dimorphisms for every neuron type in the C. elegans nervous system, with these Aims: Aim 1) Build and analyze a gene expression atlas for every neuron type at each stage of the developmental lineage. Aim 2) Develop a gene expression atlas across sexes, identifying sexually dimorphic gene expression in sex-shared neurons, as well as gene expression in sex-specific neurons. Aim 3) Describe splicing patterns for all genes and all neuron types, and use this information to identify all instances of differential alternative splicing. Aim 4) Generate and distribute analysis tools and data to the scientific community. CeNGEN represents a paradigmatic advance in neurogenetics, and provides a unique opportunity to elucidate the global control of neuron-specific gene expression and to relate developmental gene expression and splicing to ultimate neuronal wiring and function. CeNGEN will also serve as a resource for future studies in C. elegans neuroscience, and will provide a framework for addressing global differential gene expression in more complex nervous systems that are currently not amenable to this comprehensive approach.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $1,194,089 )
2026	2026	YALE UNIV	150 MUNSON ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	9	1/31/2026	NON-COMPETING CONTINUATION	$1,194,089
														Subtotal = $1,194,089

Issue Date FY: 2025 ( Subtotal = $1,194,089 )
2025	2025	YALE UNIV	150 MUNSON ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	8	1/9/2025	NON-COMPETING CONTINUATION	$1,194,089
														Subtotal = $1,194,089

Issue Date FY: 2024 ( Subtotal = $1,286,961 )
2024	2024	YALE UNIV	150 MUNSON ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	002	7	2/27/2024	NON-COMPETING CONTINUATION	$1,194,089
2024	2024	YALE UNIV	150 MUNSON ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	003	7	5/3/2024	NON-COMPETING CONTINUATION	$92,872
2024	2023	YALE UNIV	150 MUNSON ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	6	2/1/2024	COMPETING CONTINUATION	$17,601
2024	2021	YALE UNIV	150 MUNSON ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	5	2/1/2024	NON-COMPETING CONTINUATION	-$17,601
														Subtotal = $1,286,961

Issue Date FY: 2023 ( Subtotal = $1,353,606 )
2023	2023	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	6	12/28/2022	COMPETING CONTINUATION	$1,353,606
2023	2021	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	5	12/22/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $1,353,606

Issue Date FY: 2021 ( Subtotal = $1,242,113 )
2021	2021	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	5	7/29/2021	NON-COMPETING CONTINUATION	$1,242,113
														Subtotal = $1,242,113

Issue Date FY: 2020 ( Subtotal = $1,242,113 )
2020	2020	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	4	7/30/2020	NON-COMPETING CONTINUATION	$1,242,113
2020	2019	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	2/21/2020	NON-COMPETING CONTINUATION	$0
														Subtotal = $1,242,113

Issue Date FY: 2019 ( Subtotal = $1,242,113 )
2019	2019	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	3	8/7/2019	NON-COMPETING CONTINUATION	$1,242,113
2019	2018	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	12/7/2018	NON-COMPETING CONTINUATION	$0
														Subtotal = $1,242,113

Issue Date FY: 2018 ( Subtotal = $1,242,113 )
2018	2018	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	7/25/2018	NON-COMPETING CONTINUATION	$1,242,113
														Subtotal = $1,242,113

Issue Date FY: 2017 ( Subtotal = $1,112,812 )
2017	2017	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	9/21/2017	NEW	$1,112,812
														Subtotal = $1,112,812

Grand Total All Awards = $11,110,009

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Discovery and analysis of the C. elegans neuronal gene expression network (CeNGEN)

Award Number: R01NS100547

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/25/2017

PERIOD OF PERFORMANCE END DATE: 12/31/2027

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