Wednesday, December 4, 2024
12/4/2024
PROJECT ABSTRACT Glial cells of the central nervous system (CNS), such as astrocytes and microglia, are intimately involved in tuning neural circuit activity, which is crucial in brain areas where increased activity leads aberrant behavior. The amygdala is one such limbic brain area that encodes associations between environmental stimuli and fear, and fires only if a threat is detected. The basolateral amygdala (BLA) is the amygdala input nucleus that integrates processed sensory information and initiates fear responses. However, pro-inflammatory signals induced in response to recurrent environmental threats boost BLA activity and increase fear responses. Glial cells in the amygdala are integral for inhibiting inflammatory signals that otherwise drive neural activity and fear behavior. Yet, many pathways in glial cells that tune BLA immune responses underlying fear are unknown. This lack of knowledge is largely due to an absence of methods that profile the mechanisms regulating cross-talk among glial cells at high throughput. Recently, I developed a new forward genetic screening platform to profile molecules controlling cell-cell interactions called SPEAC-seq (Systematic Perturbation of Encapsulated Associated Cells followed by SEQuencing). SPEAC-seq permits the co-encapsulation of cell pairs from differing cell classes within picoliter droplets (e.g., astrocytes and microglia), where prior to encapsulation one cell type expresses an inducible state-dependent fluorescent reporter and the other cell type is randomly perturbed with an sgRNA from a genome-wide CRISPR/Cas9 library. Cell pairs are encapsulated, co-cultured, and then sorted in droplets at high throughput. Isolation of sgRNA sequences from droplets containing an activated cell permits the association of molecular regulators with cellular states. My preliminary SPEAC-seq data uncovered amphiregulin (AREG) in microglia as a negative regulator of pro-inflammatory NF-kB signals in astrocytes by signaling through EGFR. Interestingly, microglia-astrocyte AREG-EGFR signals negatively regulated expression of the BLA-restricted transcription factor ETV1 in astrocytes. In preliminary studies, I found that ETV1 drove pro-inflammatory NF-kB signaling in astrocytes and was induced in response to chronic stress in the BLA. Chemogenetic activation of BLA astrocytes boosted helplessness behavior following chronic stress while genetic perturbation of Areg in BLA microglia or Egfr in BLA astrocytes exacerbated fear responses following chronic stress. Together, these data suggest that AREG+ microglia limit the activation of NF-kB in EGFR+ astrocytes by suppressing ETV1 and stress-induced fear behavior. In this proposal, I hypothesize that AREG+ microglia limit ETV1-driven NF-kB activation in EGFR+ BLA astrocytes to decrease stress-induced fear behavior. In Aim 1, I will test the role of AREG+ BLA microglia in limiting astrocyte ETV1 expression and NF-kB signals. In Aim 2, I will uncover the transcriptional and epigenetic networks of ETV1 and its family members in BLA astrocytes by multi-omic Perturb- seq. In Aim 3, I will determine how acute or chronic activation of ETV1+ astrocytes controls fear behavior. IN SUM, this R01 proposal will study how an anti-inflammatory cell circuit in the BLA shapes stress-induced fear.
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