Wednesday, June 18, 2025 6/18/2025

SINGLE CELL GENOMICS OF HUMAN MICROBIAL FLORA

Award Number: R01HG003647
ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/12/2005
PERIOD OF PERFORMANCE END DATE: 04/30/2015

Group Awards By:

View Award Description

SINGLE CELL GENOMICS OF HUMAN MICROBIAL FLORA - DESCRIPTION (provided by applicant): This renewal application proposes to continue our work to develop the multiple displacement amplification (MDA) reaction which is used for whole genome amplification. MDA can be used to obtain amplified DNA from single cells for use in sequencing. The study will continue to focus on microbes inhabiting the human body. New methods will be demonstrated for amplifying DNA from single cells of uncultured bacteria obtained from human clinical specimens including skin, throat and fecal samples. Basic research will continue into the enzymology of the MDA reaction in order to reduce amplification bias. Microcolony technology will also be used to reduce amplification bias by providing DNA template from multiple cells rather than a single cell. Research will continue into better methods to isolate individual cell by flow cytometry and micromanipulation. Fluorescent in situ hybridization (FISH) probes will be tested for isolating specific microbes of interest. These methods will be introduced into our program to supply amplified genomic DNA for sequencing by the NIH Human Microbiome Project. There will also be increased emphasis on medically important questions including analysis of the normal bacterial community colonizing humans and pathogens involved in hospital acquired infections. We will demonstrate single cell methods for investigating a GI tract pathogen, C. difficile, that causes hospital acquired infections (in collaboration with the University of California at San Diego Medical Center). Methods to sequence uncultivated viruses and eukaryotes will also be introduced. Improved informatic methods to assemble sequences derived from MDA reactions will be developed in collaboration with Illumina Inc. and Pavel Pevzner, UCSD.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2013 ( Subtotal = $430,666 )
2013	2013	J CRAIG VENTER INSTITUTE INC	9704 MEDICAL CENTER DRIVE	ROCKVILLE	MD	20850	MONTGOMERY	USA	Human Genome Research	000	10	5/13/2013	NON-COMPETING CONTINUATION	$430,666
														Subtotal = $430,666

Issue Date FY: 2012 ( Subtotal = $450,959 )
2012	2012	J CRAIG VENTER INSTITUTE INC	9704 MEDICAL CENTER DRIVE	ROCKVILLE	MD	20850	MONTGOMERY	USA	Human Genome Research	000	9	4/28/2012	NON-COMPETING CONTINUATION	$450,959
														Subtotal = $450,959

Issue Date FY: 2011 ( Subtotal = $469,087 )
2011	2011	J CRAIG VENTER INSTITUTE INC	9704 MEDICAL CENTER DRIVE	ROCKVILLE	MD	20850	MONTGOMERY	USA	Human Genome Research	001	8	7/28/2011	COMPETING CONTINUATION	$46,909
2011	2011	J CRAIG VENTER INSTITUTE INC	9704 MEDICAL CENTER DRIVE	ROCKVILLE	MD	20850	MONTGOMERY	USA	Human Genome Research	000	8	4/27/2011	COMPETING CONTINUATION	$422,178
														Subtotal = $469,087

Issue Date FY: 2010 ( Subtotal = $393,860 )
2010	2010	J CRAIG VENTER INSTITUTE INC	9704 MEDICAL CENTER DRIVE	ROCKVILLE	MD	20850	MONTGOMERY	USA	Human Genome Research	000	7	5/28/2010	NON-COMPETING CONTINUATION	$393,860
														Subtotal = $393,860

Issue Date FY: 2009 ( Subtotal = $386,251 )
2009	2009	J CRAIG VENTER INSTITUTE INC	9704 MEDICAL CENTER DRIVE	ROCKVILLE	MD	20850	MONTGOMERY	USA	Human Genome Research	000	6	5/13/2009	NON-COMPETING CONTINUATION	$386,251
														Subtotal = $386,251

Issue Date FY: 2008 ( Subtotal = $375,000 )
2008	2008	J CRAIG VENTER INSTITUTE INC	9704 MEDICAL CENTER DRIVE	ROCKVILLE	MD	20850	MONTGOMERY	USA	Human Genome Research	000	5	5/30/2008	COMPETING CONTINUATION	$375,000
														Subtotal = $375,000

Issue Date FY: 2007 ( Subtotal = $367,181 )
2007	2007	J CRAIG VENTER INSTITUTE INC	9704 MEDICAL CENTER DRIVE	ROCKVILLE	MD	20850	MONTGOMERY	USA	Human Genome Research	000	4	5/29/2007	OTHER REVISION	$367,181
														Subtotal = $367,181

Issue Date FY: 2006 ( Subtotal = $387,017 )
2006	2006	INSTITUTE FOR GENOMIC RESEARCH (THE)	9712 MEDICAL CENTER DRIVE	ROCKVILLE	MD	20850	MONTGOMERY	USA	Human Genome Research	000	3	9/22/2006	NON-COMPETING CONTINUATION	$387,017
														Subtotal = $387,017

Issue Date FY: 2005 ( Subtotal = $375,000 )
2005	2005	ALLEGHENY SINGER RESEARCH CORP	503 MARTINDALE STREET	PITTSBURGH	PA	15212	ALLEGHENY	USA	Human Genome Research	000	1	5/11/2005	NEW	$375,000
														Subtotal = $375,000

Grand Total All Awards = $3,635,021

Top

All Categories

About

Search

Reports

Data Submission

Award Information

SINGLE CELL GENOMICS OF HUMAN MICROBIAL FLORA

Award Number: R01HG003647

ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/12/2005

PERIOD OF PERFORMANCE END DATE: 04/30/2015

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer