Sunday, June 22, 2025 6/22/2025

Regulation of Appendage Regeneration in Zebrafish

Award Number: R01HD105033
ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 06/15/2021
PERIOD OF PERFORMANCE END DATE: 05/31/2026

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Regulation of Appendage Regeneration in Zebrafish - Adult teleost fish and urodele amphibians can regenerate entire amputated appendages. By contrast, regenerative healing of adult mammalian limbs is limited to the very tips of digits. One of the key challenges in developmental biology is to understand how and why tissue regeneration occurs. The hallmark of limb or fin regeneration is formation of a blastema, a mesenchymal structure that contains progenitor cells for new skeletal elements. As regeneration proceeds, blastemal cell proliferation and patterning are regulated such that lost tissues of correct size and shape are replaced. While the catalogue of defined cell dynamics and molecular factors in tissue regeneration is expanding, we know much less of how genes involved in regenerative events are engaged upon injury. That is, what are the DNA sequences that recruit activators (or repressors) of gene programs during tissue regeneration, how are these sequences distributed throughout the genomes of regeneration-competent animals, and what are the transcription factors that engage with these sequences? Recently, we identified a class of distal gene regulatory elements that preferentially activate gene expression during regeneration, can be engineered in simple constructs to express developmental factors that promote regeneration, and have the potential to be recognized by the transcriptional machinery of distant species. The overall goal of this proposal is to discover gene regulatory concepts and mechanisms that restore size and pattern to an amputated appendage. We will test the hypothesis that enhancer and silencer elements are crucial regulatory modules enabling appendage regeneration in zebrafish. This work will increase understanding of developmental regulation during vertebrate tissue regeneration, and provide important perspective for comprehending, and perhaps changing, the existing limitations in regenerative capacity of human tissues.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $437,889 )
2025	2025	THE MORGRIDGE INSTITUTE FOR RESEARCH, INC.	330 N ORCHARD ST	MADISON	WI	53715	DANE	USA	Child Health and Human Development Extramural Research	005	6	5/13/2025	NON-COMPETING CONTINUATION	$427,145
2025	2024	DUKE UNIVERSITY	2200 W MAIN ST	DURHAM	NC	27705	DURHAM	USA	Child Health and Human Development Extramural Research	003	4	3/28/2025	NON-COMPETING CONTINUATION	$3,296
2025	2024	THE MORGRIDGE INSTITUTE FOR RESEARCH, INC.	330 N ORCHARD ST	MADISON	WI	53715	DANE	USA	Child Health and Human Development Extramural Research	002	5	3/24/2025	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
2025	2024	DUKE UNIVERSITY	2200 W MAIN ST	DURHAM	NC	27705	DURHAM	USA	Child Health and Human Development Extramural Research	006	4	6/11/2025	NON-COMPETING CONTINUATION	$0
2025	2024	THE MORGRIDGE INSTITUTE FOR RESEARCH, INC.	330 N ORCHARD ST	MADISON	WI	53715	DANE	USA	Child Health and Human Development Extramural Research	007	5	6/11/2025	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
2025	2024	THE MORGRIDGE INSTITUTE FOR RESEARCH, INC.	330 N ORCHARD ST	MADISON	WI	53715	DANE	USA	Child Health and Human Development Extramural Research	004	5	3/28/2025	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	-$3,296
2025	2024	DUKE UNIVERSITY	2200 W MAIN ST	DURHAM	NC	27705	DURHAM	USA	Child Health and Human Development Extramural Research	000	4	3/20/2025	NON-COMPETING CONTINUATION	-$90,807
2025	2024	THE MORGRIDGE INSTITUTE FOR RESEARCH, INC.	330 N ORCHARD ST	MADISON	WI	53715	DANE	USA	Child Health and Human Development Extramural Research	001	5	3/20/2025	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$101,551
														Subtotal = $437,889

Issue Date FY: 2024 ( Subtotal = $378,239 )
2024	2024	DUKE UNIVERSITY	2200 W MAIN ST	DURHAM	NC	27705	DURHAM	USA	Child Health and Human Development Extramural Research	000	4	5/22/2024	NON-COMPETING CONTINUATION	$378,239
														Subtotal = $378,239

Issue Date FY: 2023 ( Subtotal = $382,059 )
2023	2023	DUKE UNIVERSITY	2200 W MAIN ST	DURHAM	NC	27705	DURHAM	USA	Child Health and Human Development Extramural Research	000	3	3/23/2023	NON-COMPETING CONTINUATION	$382,059
														Subtotal = $382,059

Issue Date FY: 2022 ( Subtotal = $382,059 )
2022	2022	DUKE UNIVERSITY	2200 W MAIN ST STE 710	DURHAM	NC	27708	DURHAM	USA	Child Health and Human Development Extramural Research	000	2	5/9/2022	NON-COMPETING CONTINUATION	$382,059
														Subtotal = $382,059

Issue Date FY: 2021 ( Subtotal = $382,059 )
2021	2021	DUKE UNIVERSITY	2200 W MAIN ST STE 710	DURHAM	NC	27708	DURHAM	USA	Child Health and Human Development Extramural Research	000	1	6/14/2021	NEW	$382,059
														Subtotal = $382,059

Grand Total All Awards = $1,962,305

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Regulation of Appendage Regeneration in Zebrafish

Award Number: R01HD105033

ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 06/15/2021

PERIOD OF PERFORMANCE END DATE: 05/31/2026

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