Sunday, April 28, 2024
4/28/2024
Project Summary. Direct exposure to sunlight subjects cells to potentially damaging solar radiation. For superficial tissues such as the cornea, retina, and skin this is unavoidable. Biological circadian rhythms have evolved as a way to anticipate sunlight: by synchronizing cellular and behavioral rhythms to the 24-hour solar cycle, or “photoentrainment”. Most tissues in mammals contain an autonomous molecular circadian clock, and many of these are synchronized by the brain's central clock, the suprachiasmatic nucleus (SCN). However, we have found that some tissues located near the body's surface, such as skin and retina, have an ability to directly synchronize their local circadian clocks to short-wavelength light using the opsin, Opn5. In the case of retina and skin clocks, rhythmic environmental light dominates systemic circadian synchronization cues. The central hypothesis of this work is that opsins in cells typically exposed to sunlight respond to short-wavelength light, causing the release of diffusible signals which synchronize adjacent circadian clocks. This allows tissues to maintain a phase relationship with environmental light regardless of the phase of the animal's behavior. The specific aims of this proposal are to identify the intracellular molecular mechanism Opn5 uses to signal the presence of light using skin and retina as model circadian systems (Aim 1), identify the nature of diffusible signaling by which Opn5-cells influence adjacent cells and tissues (Aim 2), and determine the physiological significance of Opn5-mediated circadian clock synchronization in vivo (Aim 3). Our approach will be to focus on skin as a model circadian tissue due to its physiologic significance, its natural exposure to light, and the relative ease of distinguishing Opn5- specific photoactivation. However, studies will be repeated in retina for analyses on the similarity of signaling mechanisms in another photoentrainable tissue. The studies will employ a mixture of in vivo, ex vivo (organotypic tissue explants), and cell culture methods. The innovation of this project lies in the measurement of direct responses of clocks within mammalian tissues to environmental light and the mechanisms opsins employ outside of the visual system. The improvements of this proposal over past work are the 1) assessment of Opn5 signaling cascades for which there is still a gap in published literature, 2) assessment of tissue-level signaling dynamics and 3) a focus on the physiologic impacts of lighting environment on tissue health.
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