Thursday, December 11, 2025 12/11/2025

Super-Multiplexed Molecular Sensing in Live Cells

Award Number: R01GM151093
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/21/2023
PERIOD OF PERFORMANCE END DATE: 06/30/2027

Group Awards By:

View Award Description

Super-Multiplexed Molecular Sensing in Live Cells - The ability to visualize simultaneously a large number of distinct molecular species with high spatiotemporal resolution is crucial for interrogating complex and dynamic biological systems but still remains a major challenge in bioimaging. This is especially true for multiplex sensing of cellular analytes including reactive species, metal ions, and a plethora of difficult-to-tag metabolites. The prevalent fluorescence microscopy is severely limited for this because of the “color barrier”. Non-linear Raman imaging techniques such as Stimulated Raman Scattering (SRS) have become an increasingly valuable bioanalytical tool by offering a much greater number of resolvable ‘colors’ based on the narrower spectral linewidth of the Raman scattering bands. However, the lack of responsiveness of current Raman ‘tagging’ technologies hinders detection of changes in concentration of species of interest over time, limiting the application of SRS to the acquisition of mostly static pictures. The goal of this project is to establish the next generation chemical toolbox and complementary instrumentation to enable high-speed, super-multiplexed monitoring of transient species and events in live cells, an important but otherwise intractable goal by other traditional optical methods. We propose to design and synthesize a library of novel responsive vibrational probes for SRS and electronic pre-resonance (epr) -SRS sensing of ions, small reactive molecules and enzymatic activity (Specific Aim 1), amenable for use with state-of-the-art SRS microscopy instrumentation. The new technology will be tested for the super-multiplexed visualization of these molecular targets in the context of ER and mitochondrial interactions and remodeling in live cells (Specific Aim 2). Successful completion of the proposed plan will establish a transformative technology that would enable concurrent, dynamic visualization of key molecular components, structures and processes with high spatiotemporal resolution. This capability is essential for gaining an integrated view of cellular networks and their crosstalk and would find wide applications in unraveling complex systems in the realm of cell biology, neurobiology, immunology, and tumor biology.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $504,315 )
2025	2025	EMORY UNIVERSITY	201 DOWMAN DR NE	ATLANTA	GA	30322	DEKALB	USA	Biomedical Research and Research Training	005	4	6/27/2025	NON-COMPETING CONTINUATION	$254,246
2025	2025	NEW YORK UNIVERSITY	70 WASHINGTON SQ S	NEW YORK	NY	10012	NEW YORK	USA	Biomedical Research and Research Training	003	2	4/30/2025	NON-COMPETING CONTINUATION	$254,246
2025	2024	NEW YORK UNIVERSITY	70 WASHINGTON SQ S	NEW YORK	NY	10012	NEW YORK	USA	Biomedical Research and Research Training	000	2	12/19/2024	NON-COMPETING CONTINUATION	-$258,420
2025	2024	EMORY UNIVERSITY	201 DOWMAN DR NE	ATLANTA	GA	30322	DEKALB	USA	Biomedical Research and Research Training	004	3	4/30/2025	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$56,195
2025	2024	NEW YORK UNIVERSITY	70 WASHINGTON SQ S	NEW YORK	NY	10012	NEW YORK	USA	Biomedical Research and Research Training	003	2	4/30/2025	NON-COMPETING CONTINUATION	-$1
2025	2024	NEW YORK UNIVERSITY	70 WASHINGTON SQ S	NEW YORK	NY	10012	NEW YORK	USA	Biomedical Research and Research Training	002	1	4/30/2025	NEW	-$1
2025	2024	EMORY UNIVERSITY	201 DOWMAN DR NE	ATLANTA	GA	30322	DEKALB	USA	Biomedical Research and Research Training	001	3	12/20/2024	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$254,244
2025	2023	NEW YORK UNIVERSITY	70 WASHINGTON SQ S	NEW YORK	NY	10012	NEW YORK	USA	Biomedical Research and Research Training	002	1	4/30/2025	NEW	-$56,194
														Subtotal = $504,315

Issue Date FY: 2024 ( Subtotal = $258,421 )
2024	2024	NEW YORK UNIVERSITY	70 WASHINGTON SQ S	NEW YORK	NY	10012	NEW YORK	USA	Biomedical Research and Research Training	001	2	7/23/2024	NON-COMPETING CONTINUATION	$258,421
2024	2023	NEW YORK UNIVERSITY	70 WASHINGTON SQ S	NEW YORK	NY	10012	NEW YORK	USA	Biomedical Research and Research Training	000	1	11/13/2023	NEW	$0
														Subtotal = $258,421

Issue Date FY: 2023 ( Subtotal = $258,421 )
2023	2023	NEW YORK UNIVERSITY	70 WASHINGTON SQ S	NEW YORK	NY	10012	NEW YORK	USA	Biomedical Research and Research Training	000	1	9/20/2023	NEW	$258,421
														Subtotal = $258,421

Grand Total All Awards = $1,021,157

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Super-Multiplexed Molecular Sensing in Live Cells

Award Number: R01GM151093

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/21/2023

PERIOD OF PERFORMANCE END DATE: 06/30/2027

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer