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Investigate the function of NDF in chromatin dynamics and gene expression

Award Number: R01GM145748
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 12/15/2022
PERIOD OF PERFORMANCE END DATE: 11/30/2027

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Investigate the function of NDF in chromatin dynamics and gene expression - Project Summary/Abstract Our current understanding of chromatin transcription has been limited by our knowledge of the factors that are involved in this process. In this regard, we have identified a novel nucleosome destabilization factor, termed NDF, that can destabilize nucleosomes and facilitate transcription elongation through nucleosomes. In this proposal, we will investigate the function of NDF in chromatin dynamics and RNA Polymerase II transcription elongation using biochemistry, genomics, bioinformatics and structural biology approaches. In Specific Aim 1, we will develop and employ new methods and strategies to study the molecular mechanism of NDF-mediated nucleosome destabilization and RNA Polymerase II transcription. We will use ultra-high-resolution optical tweezer as well as cryo-electron microscopy to characterize the dynamics of NDF-mediated nucleosome destabilization. In Specific Aim 2, we will examine how NDF is recruited to the transcribed regions of active genes in cells. We will assess whether other cellular factors, other than H3K36me3 histones, contribute to the recruitment of NDF. In Specific Aim 3, we will determine how genes lacking NDF are transcribed, and investigate protein factors that are related to NDF. The studies proposed here should significantly increase our understanding of the mechanisms of Pol II transcription elongation through chromatin.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $296,731 )
2025	2025	RUTGERS, THE STATE UNIVERSITY	3 RUTGERS PLZ	NEW BRUNSWICK	NJ	08901	MIDDLESEX	USA	Biomedical Research and Research Training	000	3	11/25/2024	NON-COMPETING CONTINUATION	$296,731
														Subtotal = $296,731

Issue Date FY: 2024 ( Subtotal = $329,700 )
2024	2024	RUTGERS, THE STATE UNIVERSITY	3 RUTGERS PLZ	NEW BRUNSWICK	NJ	08901	MIDDLESEX	USA	Biomedical Research and Research Training	001	2	6/24/2024	NON-COMPETING CONTINUATION	$32,971
2024	2024	RUTGERS, THE STATE UNIVERSITY	3 RUTGERS PLZ	NEW BRUNSWICK	NJ	08901	MIDDLESEX	USA	Biomedical Research and Research Training	000	2	11/1/2023	NON-COMPETING CONTINUATION	$296,729
														Subtotal = $329,700

Issue Date FY: 2023 ( Subtotal = $329,700 )
2023	2023	RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY	3 RUTGERS PLZA	NEW BRUNSWICK	NJ	08901	MIDDLESEX	USA	Biomedical Research and Research Training	000	1	12/15/2022	NEW	$329,700
														Subtotal = $329,700

Grand Total All Awards = $956,131

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Investigate the function of NDF in chromatin dynamics and gene expression

Award Number: R01GM145748

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 12/15/2022

PERIOD OF PERFORMANCE END DATE: 11/30/2027

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