Tuesday, November 4, 2025 11/4/2025

Photo-hyperpolarized 13C MRI

Award Number: R01GM143626
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/28/2022
PERIOD OF PERFORMANCE END DATE: 07/31/2026

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Photo-hyperpolarized 13C MRI - Summary. High resolution imaging in deep tissue (> 1 cm) environments can address a swathe of funda- mental and applied problems in the elucidation of mechanisms of disease origin and progression. While fluores- cence imaging is a workhorse technique for the cellular imaging of biological molecular markers, it suffers from light scattering, and aberration distortions at tissue depths >1 mm. On the opposite end of the spectrum, mag- netic resonance imaging (MRI) is a well-established and broadly employed pre-clinical and clinical imaging method that has no practical limitations with respect to tissue depth, but it suffers from low resolution. In this project we will innovate a new class of hyperpolarized 13C nanoparticle probes that can serve as efficient deep tissue markers in MRI. Our central idea is to dramatically boost 13C NMR signal by means of (i) optical hyperpo- larization that can be carried out at low magnetic fields and (ii) significant extension of 13C coherence times. Specifically, we propose to develop MRI probes based on fluorescent nanodiamonds (FNDs) endowed with nitrogen-vacancy (NV) centers. The electronic spins associated with NVs can be optically “hyperpolarized” and that polarization to be effectively transferred to the diamond 13C nuclear spins, resulting in NMR signal enhance- ment over three orders of magnitude vs. 13C thermal polarization at the fields of clinical MRI. In conjunction, by implementing effective decoupling schemes we propose significantly extend the 13C spin coherences to be able to interrogate them for second-long periods. The latter yields enormous signal gains, a multiplicative factor of another 103- fold. Combining the gains due to hyperpolarization and spin coherence extensions permits a total signal gain of ca. 106 for MRI, and will enable a significant improvement in spatial resolution. Moreover, since the polarization is optically generated, this 13C photo-MRI (PMRI), can be carried out at low-field at a much lower cost vs. conventional MRI infrastructure. In our method the spin polarization is regenerated optically, allowing for acquiring MRI data repeatedly and enabling longitudinal studies. Furthermore, the FND particles are inherently biocompatible, and their surfaces are amenable to a versatile set of targeting ligands. With this basis, we propose to develop targetable fluorescent nanoparticle MRI probes that can be imaged with high fidelity with resolution better than 20 um in deep tissue (>1 cm) settings. In addition to being bright MRI agents, the particles are also bright fluorescent providing an option for a cross-examination of the agent biodistribution in histopathological analysis. In order to realize the prospects of this novel technology, we propose to further develop the hyperpo- larization and MR imaging methodologies, as well as boost hyperpolarizability of nanosized particles by optimiz- ing their structure through synthesis and processing developments. We aim at transferring the PMRI technology we demonstrated for micron-sized particles to the nanosized FND suitable for in vivo MRI. As a part of the technology demonstration, we will construct a simple prototype PMRI imaging set-up on the benchtop (low-field) and image FNDs in tissue phantoms, characterizing achievable metrics of resolution and imaging depth.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $332,818 )
2025	2025	ADAMAS NANOTECHNOLOGIES, INC.	8100 BROWNLEIGH DRIVE	RALEIGH	NC	27617	WAKE	USA	Biomedical Research and Research Training	001	4	9/19/2025	NON-COMPETING CONTINUATION	$332,818
2025	2024	ADAMAS NANOTECHNOLOGIES, INC.	8100 BROWNLEIGH DRIVE	RALEIGH	NC	27617	WAKE	USA	Biomedical Research and Research Training	000	3	9/16/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $332,818

Issue Date FY: 2024 ( Subtotal = $333,773 )
2024	2024	ADAMAS NANOTECHNOLOGIES, INC.	8100 BROWNLEIGH DRIVE	RALEIGH	NC	27617	WAKE	USA	Biomedical Research and Research Training	000	3	9/17/2024	NON-COMPETING CONTINUATION	$333,773
														Subtotal = $333,773

Issue Date FY: 2023 ( Subtotal = $337,202 )
2023	2023	ADAMAS NANOTECHNOLOGIES INC	8100 BROWNLEIGH DR STE 120	RALEIGH	NC	27617	WAKE	USA	Biomedical Research and Research Training	001	2	9/6/2023	NON-COMPETING CONTINUATION	$337,202
2023	2022	ADAMAS NANOTECHNOLOGIES INC	8100 BROWNLEIGH DR STE 120	RALEIGH	NC	27617	WAKE	USA	Biomedical Research and Research Training	000	1	4/25/2023	NEW	$0
														Subtotal = $337,202

Issue Date FY: 2022 ( Subtotal = $385,632 )
2022	2022	ADAMAS NANOTECHNOLOGIES INC	8100 BROWNLEIGH DR STE 120	RALEIGH	NC	27617	WAKE	USA	Biomedical Research and Research Training	000	1	9/27/2022	NEW	$385,632
														Subtotal = $385,632

Grand Total All Awards = $1,389,425

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Photo-hyperpolarized 13C MRI

Award Number: R01GM143626

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/28/2022

PERIOD OF PERFORMANCE END DATE: 07/31/2026

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