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Three-Dimensional (3D) Acoustofluidic Scanning Nanoscope with Super Resolution and Large Field of View

Award Number: R01GM143439
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/15/2021
PERIOD OF PERFORMANCE END DATE: 07/31/2025

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Three-Dimensional (3D) Acoustofluidic Scanning Nanoscope with Super Resolution and Large Field of View - PROJECT SUMMARY Over the past two decades, a number of “super-resolution” 3D imaging technologies have been developed, enabling researchers to observe nanoscale biological structures that were previously invisible to traditional, diffraction-limited imaging techniques. The ability to visualize cellular and subcellular structures at the nanoscale has revealed key insights into a variety of biological processes. Although impressive progress has been made in the development of 3D super-resolution imaging techniques, researchers are often forced to accept a tradeoff in terms of the resolution, field-of-view, speed, and ease of use of their 3D imaging technique. Recently, we have developed an acoustofluidic scanning nanoscope that can simultaneously achieve both super-resolution and large field-of-view imaging in 2D. In this R01 project, we will develop and validate a 3D acoustofluidic scanning nanoscope with the following features: (1) Super-resolution imaging with lateral and axial resolutions of ~50 nm and ~120 nm, respectively: The proposed 3D imaging method will achieve a resolution that is four times better than that from a confocal microscope, which makes the optical imaging of more detailed inner architecture of many subcellular structures possible; (2) Large field-of-view (~1,100×1,100 µm2): Conventional optical imaging methods achieve high-throughput imaging at the cost of reduced resolution and vice versa. By utilizing acoustics to simultaneously manipulate multiple microsphere lenses, the proposed imaging method will solve this long-standing technical barrier for large field-of-view imaging while maintaining superior lateral and axial resolution; (3) Imaging speed 10 times faster than that from a confocal microscope: Rapid z-stacking at a speed 10 times faster than that of a confocal microscope can be achieved by using surface acoustic waves to scan an array of microspheres across the sample volume in a precise, controllable manner; (4) Seamless connection to a conventional optical microscope for ease of use: Our device can be seamlessly connected to a conventional optical microscope without modification of the optical setup, which can significantly reduce the cost and the complexity of operation. With the aforementioned advantages, the proposed 3D acoustofluidic scanning nanoscope technology has the potential to significantly exceed current standards in the field and address many unmet needs. We will validate its performance by imaging 3D nanorod samples and the organelles of live HeLa cells. In this regard, we aim to demonstrate the far-reaching potential of our 3D acoustofluidic scanning nanoscope technology to enable improved research in areas ranging from subcellular imaging to the visualization of 3D neural activity.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $370,593 )
2024	2024	UNIVERSITY OF DAYTON	300 COLLEGE PARK AVE	DAYTON	OH	45469	MONTGOMERY	USA	Biomedical Research and Research Training	001	4	9/9/2024	NON-COMPETING CONTINUATION	$370,593
2024	2023	UNIVERSITY OF DAYTON	300 COLLEGE PARK AVE	DAYTON	OH	45469	MONTGOMERY	USA	Biomedical Research and Research Training	000	3	4/22/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $370,593

Issue Date FY: 2023 ( Subtotal = $369,931 )
2023	2023	UNIVERSITY OF DAYTON	300 COLLEGE PARK	DAYTON	OH	45469	MONTGOMERY	USA	Biomedical Research and Research Training	000	3	7/12/2023	NON-COMPETING CONTINUATION	$369,931
														Subtotal = $369,931

Issue Date FY: 2022 ( Subtotal = $369,298 )
2022	2022	UNIVERSITY OF DAYTON	300 COLLEGE PARK	DAYTON	OH	45469	MONTGOMERY	USA	Biomedical Research and Research Training	000	2	7/25/2022	NON-COMPETING CONTINUATION	$369,298
														Subtotal = $369,298

Issue Date FY: 2021 ( Subtotal = $381,237 )
2021	2021	UNIVERSITY OF DAYTON	300 COLLEGE PARK	DAYTON	OH	45469	MONTGOMERY	USA	Biomedical Research and Research Training	000	1	8/30/2021	NEW	$381,237
														Subtotal = $381,237

Grand Total All Awards = $1,491,059

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Three-Dimensional (3D) Acoustofluidic Scanning Nanoscope with Super Resolution and Large Field of View

Award Number: R01GM143439

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/15/2021

PERIOD OF PERFORMANCE END DATE: 07/31/2025

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